Hello!, I want to see the DNA pellet, is there any dye or something to paint the pellet and see it ?
Thanks!
Martin.
Fluorescent as well as non-fluorescent additives are available from commercial sources, for example Millipore.
There is large range of dyes for DNA staining. If you need a fluorescent one, Dapi for example give very good results and it is safe. If you want a non-fluorescent one, you can use a dye targeting for example negatively charged molecules, such as crystal violet.
It is important to be careful concerning the dye choice because many DNA dyes are dangerous !
Cora, if i want to see the pellet when i do a DNA ethanol precipitation, with dye you recommend me to use ? Crystal Violet? any recommended concentration?
Many thanks!
Martin
Dear
To see a pellet when precipitating very small amounts of DNA, a co-precipitant
or carrier can be a real advantage. The type of carrier to be added will
depend on what the DNA is to be used for after precipitation. You can used Crystal violet.
Cordialy
Marius
Dear Marius, could you provide me a concentration of Crystal violet i should use?
Thanks!
Martin.
Maybe this publication can be interesting for you, here they stained DNA for electrophoresis but i think it could work to stained a pellet (with some adaptations).
http://onlinelibrary.wiley.com/doi/10.1002/bmb.2004.494032050398/full
in former times, glycogen mussle blue (but it is not colored, but is a quite inert white mass) was used to make precipitates of small amounts of DNA or RNA more easily 'visible' for eyes and at the same time to enhance precipitation. It gets solved when DNA is solved. And it practically doesn't interact with any of the downstream applications (inert). This hint I got from my doctor father.
It can be buyed here:
http://www.sigmaaldrich.com/catalog/product/sigma/g1767?lang=de®ion=DE
This vial will take very long to be used - so you practically buy it once for your entire PhD thesis time. I used it during my thesis.
I think this might be helpful for you. (Dextran blue staining of DNA pellets)
http://www.sciencedirect.com/science/article/pii/S1366212007700120
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