11 June 2021 4 10K Report

I am interested in a software/solution which will allow employees across the company to submit request for various analyses in the labs, allow the lab managers to track the samples and work load distribution. Any recommendations, suggestions are appreciated.

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What may be the effective strategy to design primers of miRNAs for qRT-PCR?

I designed Primers for miRNAs using mirPrimer algorithm. However when l analysed them by Oligoanalyzer 3.1, it shows Primer-dimer and secondary structure formation. I request your suggestion...

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Why there is continuous need to genetically engineer a yeast that is already an industrially high-yielding strain??

It is generally observed that scientists and researchers worldwide always try to genetically engineer wild-type hyper-producer (high-yielding) strains that already produce enough/excellent...

31 August 2020 4,104 3 View

Chemical absorption Co2 removal technologies?

Dear All, Can Any one list down commercially available chemical absorption technologies which can be used for absorption of CO2 from syntehsis gas

17 August 2020 627 2 View

Isolating Total RNA from Paraffin blocks by TRI reagent?

Dear All, I got 2 weeks old paraffin blocks of tumor masses. I want to extract total RNA from them. The available reagents in my lab are: 1) Cell dissociation buffer (GIBCO#13151-014) 2) TRI...

30 March 2020 5,067 5 View

Differences in Load controlled and displacement controlled conditions ?

Why is that load controlled and displacement controlled boundary conditions give different results for same meshing and boundary restraints ? I specify time at the end of load step as 1 and no of...

13 February 2019 8,670 6 View

Selection of node from a subset of nodes corresponding to maximum displacement in one direction?

How to select a node from a set of selected nodes and get the node number corresponding to maximum value of say y displacement. It should also take the mod (for selection of maximum negative...

27 January 2019 5,097 1 View

For Immunofluorescence, Which dye is suitable in secondary antibody if your cells are GFP positive?

My cells are transfected with GFP plasmid. I want to detect a particular cell surface marker by immunostaining. Can I use Alexa Fluor 488 dye containing secondary antibody? Will it overlap with...

27 September 2018 4,502 6 View

How to SELECT THE keypoints associated with area numbers in APDL?

I have selected a subset of areas by ASEL,S,LOC,Y,0 APLOT With the selected areas I have counted them via *GET,A,AREA,,COUNT then I have defined an array via *DIM,B,ARRAY,1,AREA This creates an...

22 July 2018 4,456 4 View

How to combine result of excitation and emission radiations detected by microplate reader as one reading?

I did ROS assay using H2-DCFDA but could not find the function of detecting 2 wavelengths ( ex: 485, em: 520) at the same time as single reading in microplate reader so I got readings separately...

01 July 2018 6,590 5 View

Does heat Inactivation surely inactivate the restriction enzymes?

After digesting vector with AsiSI and NotI, I gel purified and quantified. The insert was PCR amplified, gel purified and cut with same enzymes. After cutting insert, I did not gel purify but heat...

04 June 2018 8,682 7 View

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