How to validate image labeling quality within dataset?

Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is

18 February 2021 4,254 5 View

Are there any DNA extraction kit from resins?

Hi everyone, I'm looking for a DNA extraction kit or some protocols to obtain the total DNA from resin samples. For this purpose, some studies suggest the use of plant extraction protocol of...

25 October 2020 3,898 3 View

How to calculate the effect size of a mixed models anova (from existing research papers)?

Hi all I'm comparing some research papers for my university. The most studies use a mixed model ANOVA design, but did not specify the effect sizes. 1. Is it possible to (re)calculate the effects...

23 October 2020 5,013 3 View

Antibody : loss of binding at high concentration (beyond saturation) Why?

Hi everyone, It is commonly observed that some antibodies presents a binding saturation curve that goes down at high concentration (at concentration beyond saturation). Does anybody have a good...

20 August 2020 778 5 View

TRP1 deficient yeast strain not growing on 5FAA containing medium?

05 August 2020 3,010 5 View

What would be the ideal number of epoch for my Neural Network?

Hello, If I am training my Neural Network with training data comprised of 908 batches, as the rule of thumb, would 20 epochs for training be sufficient? If 20 epochs are not sufficient, does...

26 March 2020 2,291 6 View

How important is it to set seed in experiments in computer science?

Hello, If I am going to submit my work to a CS conference, and also make my Python code available publicly, is it absolutely necessary that I set seed in my Python code? I ran an experiment, but...

07 March 2020 1,832 3 View

How to process ARC dataset with GTP2 Double Heads Model?

Hello, I am interested in processing the ARC dataset (http://nlpprogress.com/english/question_answering.html) with the GPT2 double heads model neural network. The dataset (tab delimited) is...

22 November 2019 3,796 1 View

How to distinguish "elementary English" from "advanced English"?

Hello, When we take a look at English test prep books that are specially designed for ESL learners, we often see in their title that the book is for "learners at elementary level", "learners at...

15 November 2019 5,393 1 View

Can someone please guide me to a software that I can use to create time lapse models for the marine environment?

I am working on a concept related to the formation of coral reefs and I need to demonstrate the concept in the form of a visual model that changes over time. I am fairly new to this area of...

16 November 2018 9,276 4 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View