How to treat/enzyme digest human tonsils for isolation leucocytes?

We want to phenotype all leucocyte populations in human tonsils by multi-color flow cytometry. Do we need an enzymatic treatment for getting out all the leucocytes (DCs, Plasmacells etc.)? If so...

08 September 2018 7,182 2 View

Advise on protocol and antibody to stain human CD4 T cells for TGF beta1?

I want to stain human activated (7 days culture) PBMCs for intracellular TGFbeta. What is the best clone and protocol. Thanks

08 September 2018 4,811 0 View

Anybody knows if an antibody against Human ST2/IL-33R that is fluorchrome- conjugated is available ?

I want to determine expression on different Human PBMC populations, cannot find it through my search on the internet. I only found a polyclonal non-conjugated anti-human ST2 from R&D. Or does...

09 October 2017 8,888 6 View

What source of serum is used for human autologous T and B cell cocultures for assessing antigen-specific follicular helper T cell function?

05 June 2017 5,936 3 View

Is there any indication of inflammasome regulation by regulatory T cells?

There are no articles describing intensive research in this field except one that mentions that only memory (mouse) T cells suppress NLRP3 activation, but not Tregs. Does anybody know some more?...

03 April 2014 4,433 0 View

What is the immune cells composition in percentage of PBMC's isolated from children (>5 yrs.)?

I want to have an idea about the percentage of CD3+ T cells present.

01 February 2014 3,354 2 View

At what point do you add Brefeldin A when stimulating regulatory T cells and Gamma Delta T cells for cytokine detection by flow?

I have used this method before with mice T cells for detecting cytokines and added Bref.A immediately after PMA/Ionomyc. but in the literature there is a lot of differences in the time of...

01 February 2014 1,294 23 View

Does anybody have experience with Invivoscribe IGH Somatic Hypermutation assay?

I want to use it for CLL analysis.

10 November 2013 2,250 0 View

Which FC-block do you use for staining human PBMC's?

Seeking to stain T cells for surface-markers and ICC for cytokines/Foxp3, do I need to use FC-bock before surface-staining and what is recommended? (eg. Heat-inactivated human serum). I do not...

08 September 2013 3,841 7 View

Can I freeze human PBMCs for intracellular Foxp3/cytokine staining?

Due to logistics, I might need to freeze the PBMCs isolated after Ficoll-paque stimulation and then thaw later and perform intracellular cytokine staining of regulatory T cells and gamma delta T...

05 June 2013 7,928 27 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View