Alberta liesbeth Paul

12 Questions 17 Answers 0 Followers

Questions related from Alberta liesbeth Paul

Advise on protocol and antibody to stain human CD4 T cells for TGF beta1?

I want to stain human activated (7 days culture) PBMCs for intracellular TGFbeta. What is the best clone and protocol. Thanks

09 September 2018 4,879 0 View

How to treat/enzyme digest human tonsils for isolation leucocytes?

We want to phenotype all leucocyte populations in human tonsils by multi-color flow cytometry. Do we need an enzymatic treatment for getting out all the leucocytes (DCs, Plasmacells etc.)? If so...

09 September 2018 7,246 2 View

Anybody knows if an antibody against Human ST2/IL-33R that is fluorchrome- conjugated is available ?

I want to determine expression on different Human PBMC populations, cannot find it through my search on the internet. I only found a polyclonal non-conjugated anti-human ST2 from R&D. Or does...

10 October 2017 8,943 6 View

What source of serum is used for human autologous T and B cell cocultures for assessing antigen-specific follicular helper T cell function?

We will assess viral-antigen function in PBMCs after virus challenge. There is discussion of adding human AB serum instead of FBS or autologous serum. Any comments, taking in mind factors in...

06 June 2017 6,047 3 View

Is there any indication of inflammasome regulation by regulatory T cells?

There are no articles describing intensive research in this field except one that mentions that only memory (mouse) T cells suppress NLRP3 activation, but not Tregs. Does anybody know some more?...

04 April 2014 4,497 0 View

What is the immune cells composition in percentage of PBMC's isolated from children (>5 yrs.)?

I want to have an idea about the percentage of CD3+ T cells present.

02 February 2014 3,410 2 View

At what point do you add Brefeldin A when stimulating regulatory T cells and Gamma Delta T cells for cytokine detection by flow?

I have used this method before with mice T cells for detecting cytokines and added Bref.A immediately after PMA/Ionomyc. but in the literature there is a lot of differences in the time of...

02 February 2014 1,385 23 View

Does anybody have experience with Invivoscribe IGH Somatic Hypermutation assay?

I want to use it for CLL analysis.

11 November 2013 2,301 0 View

Which FC-block do you use for staining human PBMC's?

Seeking to stain T cells for surface-markers and ICC for cytokines/Foxp3, do I need to use FC-bock before surface-staining and what is recommended? (eg. Heat-inactivated human serum). I do not...

09 September 2013 3,912 7 View

Can I freeze human PBMCs for intracellular Foxp3/cytokine staining?

Due to logistics, I might need to freeze the PBMCs isolated after Ficoll-paque stimulation and then thaw later and perform intracellular cytokine staining of regulatory T cells and gamma delta T...

06 June 2013 8,018 27 View

What is the optimal protocol for detecting IL-17A/IL-10 in short-term cultured (from hrs to ON) gamma delta T cells from human PB ?

I want to investigate cytokines produced by human gamma delta T cells by flow cytometry in PBMCs after isolation by microbeads. My stimulus would be PMA and ionomycine followed by inhibition by...

05 May 2013 343 3 View

Is there any way to measure mevalonate pathway metabolites by flow cytometry in human monocytes

We wonder whether inflammasome activation results in modulation of the mevalonate pathway (especially IPP levels). Reports have described the effect of mevalonate pathway on triggering...

05 May 2013 9,953 0 View