How to treat/enzyme digest human tonsils for isolation leucocytes?

We want to phenotype all leucocyte populations in human tonsils by multi-color flow cytometry. Do we need an enzymatic treatment for getting out all the leucocytes (DCs, Plasmacells etc.)? If so...

08 September 2018 7,182 2 View

Anybody knows if an antibody against Human ST2/IL-33R that is fluorchrome- conjugated is available ?

I want to determine expression on different Human PBMC populations, cannot find it through my search on the internet. I only found a polyclonal non-conjugated anti-human ST2 from R&D. Or does...

09 October 2017 8,888 6 View

What source of serum is used for human autologous T and B cell cocultures for assessing antigen-specific follicular helper T cell function?

05 June 2017 5,936 3 View

Is there any indication of inflammasome regulation by regulatory T cells?

There are no articles describing intensive research in this field except one that mentions that only memory (mouse) T cells suppress NLRP3 activation, but not Tregs. Does anybody know some more?...

03 April 2014 4,433 0 View

What is the immune cells composition in percentage of PBMC's isolated from children (>5 yrs.)?

I want to have an idea about the percentage of CD3+ T cells present.

01 February 2014 3,354 2 View

At what point do you add Brefeldin A when stimulating regulatory T cells and Gamma Delta T cells for cytokine detection by flow?

I have used this method before with mice T cells for detecting cytokines and added Bref.A immediately after PMA/Ionomyc. but in the literature there is a lot of differences in the time of...

01 February 2014 1,294 23 View

Does anybody have experience with Invivoscribe IGH Somatic Hypermutation assay?

I want to use it for CLL analysis.

10 November 2013 2,250 0 View

Which FC-block do you use for staining human PBMC's?

Seeking to stain T cells for surface-markers and ICC for cytokines/Foxp3, do I need to use FC-bock before surface-staining and what is recommended? (eg. Heat-inactivated human serum). I do not...

08 September 2013 3,841 7 View

Can I freeze human PBMCs for intracellular Foxp3/cytokine staining?

Due to logistics, I might need to freeze the PBMCs isolated after Ficoll-paque stimulation and then thaw later and perform intracellular cytokine staining of regulatory T cells and gamma delta T...

05 June 2013 7,928 27 View

What is the optimal protocol for detecting IL-17A/IL-10 in short-term cultured (from hrs to ON) gamma delta T cells from human PB ?

I want to investigate cytokines produced by human gamma delta T cells by flow cytometry in PBMCs after isolation by microbeads. My stimulus would be PMA and ionomycine followed by inhibition by...

04 May 2013 276 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View