How to treat/enzyme digest human tonsils for isolation leucocytes?

We want to phenotype all leucocyte populations in human tonsils by multi-color flow cytometry. Do we need an enzymatic treatment for getting out all the leucocytes (DCs, Plasmacells etc.)? If so...

08 September 2018 7,182 2 View

Advise on protocol and antibody to stain human CD4 T cells for TGF beta1?

I want to stain human activated (7 days culture) PBMCs for intracellular TGFbeta. What is the best clone and protocol. Thanks

08 September 2018 4,811 0 View

What source of serum is used for human autologous T and B cell cocultures for assessing antigen-specific follicular helper T cell function?

05 June 2017 5,936 3 View

Is there any indication of inflammasome regulation by regulatory T cells?

There are no articles describing intensive research in this field except one that mentions that only memory (mouse) T cells suppress NLRP3 activation, but not Tregs. Does anybody know some more?...

03 April 2014 4,433 0 View

What is the immune cells composition in percentage of PBMC's isolated from children (>5 yrs.)?

I want to have an idea about the percentage of CD3+ T cells present.

01 February 2014 3,354 2 View

At what point do you add Brefeldin A when stimulating regulatory T cells and Gamma Delta T cells for cytokine detection by flow?

I have used this method before with mice T cells for detecting cytokines and added Bref.A immediately after PMA/Ionomyc. but in the literature there is a lot of differences in the time of...

01 February 2014 1,294 23 View

Does anybody have experience with Invivoscribe IGH Somatic Hypermutation assay?

I want to use it for CLL analysis.

10 November 2013 2,250 0 View

Which FC-block do you use for staining human PBMC's?

Seeking to stain T cells for surface-markers and ICC for cytokines/Foxp3, do I need to use FC-bock before surface-staining and what is recommended? (eg. Heat-inactivated human serum). I do not...

08 September 2013 3,841 7 View

Can I freeze human PBMCs for intracellular Foxp3/cytokine staining?

Due to logistics, I might need to freeze the PBMCs isolated after Ficoll-paque stimulation and then thaw later and perform intracellular cytokine staining of regulatory T cells and gamma delta T...

05 June 2013 7,928 27 View

What is the optimal protocol for detecting IL-17A/IL-10 in short-term cultured (from hrs to ON) gamma delta T cells from human PB ?

I want to investigate cytokines produced by human gamma delta T cells by flow cytometry in PBMCs after isolation by microbeads. My stimulus would be PMA and ionomycine followed by inhibition by...

04 May 2013 276 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View