I want to use it for CLL analysis.
We want to phenotype all leucocyte populations in human tonsils by multi-color flow cytometry. Do we need an enzymatic treatment for getting out all the leucocytes (DCs, Plasmacells etc.)? If so...
08 September 2018 7,276 2 View
I want to stain human activated (7 days culture) PBMCs for intracellular TGFbeta. What is the best clone and protocol. Thanks
08 September 2018 4,902 0 View
I want to determine expression on different Human PBMC populations, cannot find it through my search on the internet. I only found a polyclonal non-conjugated anti-human ST2 from R&D. Or does...
09 October 2017 8,962 6 View
We will assess viral-antigen function in PBMCs after virus challenge. There is discussion of adding human AB serum instead of FBS or autologous serum. Any comments, taking in mind factors in...
05 June 2017 6,074 3 View
There are no articles describing intensive research in this field except one that mentions that only memory (mouse) T cells suppress NLRP3 activation, but not Tregs. Does anybody know some more?...
03 April 2014 4,523 0 View
I want to have an idea about the percentage of CD3+ T cells present.
01 February 2014 3,434 2 View
I have used this method before with mice T cells for detecting cytokines and added Bref.A immediately after PMA/Ionomyc. but in the literature there is a lot of differences in the time of...
01 February 2014 1,414 23 View
Seeking to stain T cells for surface-markers and ICC for cytokines/Foxp3, do I need to use FC-bock before surface-staining and what is recommended? (eg. Heat-inactivated human serum). I do not...
08 September 2013 3,947 7 View
Due to logistics, I might need to freeze the PBMCs isolated after Ficoll-paque stimulation and then thaw later and perform intracellular cytokine staining of regulatory T cells and gamma delta T...
05 June 2013 8,046 27 View
I want to investigate cytokines produced by human gamma delta T cells by flow cytometry in PBMCs after isolation by microbeads. My stimulus would be PMA and ionomycine followed by inhibition by...
04 May 2013 371 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
