I have cryopreserved caco2 cell line and the previous batch of cells had yeast contamination when cultured. Is there any procedure/washing that can be followed to remove the yeast contamination from the cryopreserved caco2 cells before culturing them (growing them again). I have just one back up cells with me and your guidance/suggestions are vital for me. The medium I have used contains 10% FCS, 1% Glutamine, 1% PEN-STREP, 1% NEAA.
I have never heard of a way to remove yeast. I suggest you replace your cells with fresh stock and pay strict attention to technique with your new cells. Either replace your reagents or test them for sterility before you continue. Good luck.
Hi all, I was following this question in hopes that there could be some way to get rid of yeast contamination in my cell culture.
I tried Gentamcin-Amphotericin B solutions from ATCC, but that just slowed them down a bit and made them more angry when they grew back.
I tossed the cell flasks out before the yeast were able to make beer in my incubator.
But seriously, is there no way to kill yeast without killing mammalian cells?.
I agree with all the comments so far. Once contaminated with yeast, it is better to get rid that entire batch of cells. Even if you find a way to clean it, it is always risky to use that cell. Yeast grows tens of folds faster than Caco-2 cells. As such cell lines behave differently at different passage and at different state of differentiation. Addition of another variable is certainly not a good idea if you want to generate reproducible data.
hello
i do agree with the answers above. my lab had a phoenix cells for generating virus. however, it was contaminated with yeast. so i tried nyastatin, amphotericin B but it couldn't help much. i had to rely on other means. so i agree that to save yourself, it will be best to get a fresh fungi free vial.
It can't be saved. The best thing is to discard the contaminated-yeast cells. Purchase the new one
The best way to work is to start from a new batch of cells ( not contaminated with yeast) or buy new one
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