Behin Sundara Raj

10 Questions 15 Answers 0 Followers

Questions related from Behin Sundara Raj

I am detecting singlet oxygen by DPBF using RoseBengal as standard, ethanol as solvent. Does DPBF/RB and Samples have a fixed concentration tobe used?

I have couple of queries related to SINGLET OXYGEN detection by DPBF: On what basis the concentration for DPBF/ Rose Bengal and samples are fixed?Do they (RB/DPBFand samples) have a fixed...

06 June 2016 5,757 1 View

Is there any standard procedure for removing yeast contamination from cryo preserved caco2 cell line before growing them?

I have cryopreserved caco2 cell line and the previous batch of cells had yeast contamination when cultured. Is there any procedure/washing that can be followed to remove the yeast contamination...

12 December 2014 6,344 7 View

Do Folate receptors express well in late passage of Caco2 cells?

I am working on Caco2 cells and interested in the folate receptors which are highly expressed in the cells. I am using a late passage (90), where cells are growing fine, but can someone...

12 December 2014 4,616 2 View

Can anyone share your experience of how stable nanoparticles were in different mediums over time?

I am studying the stability of nanoparticles at 37 degree for 3 days in different medium like water, PBS. Do they agglomerate to micrometer range?

11 November 2014 7,827 9 View

How to detect singlet oxygen, superoxide radical by diphenylisobenzofuran (DPBF)?

I will be using DPBF for singlet oxygen detection, caused by nanoparticles in my study. Anyone who has used the method or followed a reference article, could you please provide me the details,...

10 October 2014 6,275 2 View

Does anyone know about distributors/designs of a UV chamber that can be used for both in vitro (cell culture) and in vivo (animal) studies?

I will be using UV radiation as part of my work in cell culture and animal studies. I would like to know if there is an UV chamber/design available that can be used for both purposes.

09 September 2014 7,945 5 View

Can anyone provide me information about UV lamp and UV chamber suppliers, reasonable in cost within Australia?

I am in need of UV lamps (UVA/UVB/UVC) and UV chamber that can be used inside a biosafety cabinet for cell culture studies. The lamp needs to be compact and the chamber needs to be a closed one,...

09 September 2014 3,050 2 View

1,3-diphenylisobenzofuran(DPBF) is used to quantify singlet oxygen production, can we perform the same procedure in cell lines also?

I will be measuring the fluorescence of DPBF in singlet oxygen detection for its quantification. Can the same procedure be followed for measuring singlet oxygen generated in cell lines?

08 August 2014 1,619 5 View

Can anyone help me in designing a safe method for UV light exposure(from lamp) to cell cultures?

I need a safe procedure as it involves radiation. I am working on PDT, which involves UV light exposure to cell cultures. I need to know a safe, simple procedure to do the same. The source of...

08 August 2014 7,986 3 View

Is it possible to determine the amount of Zinc present in the blood of mice other than ICP-AES/ ICP-MS techniques

I am planning to determine the amount of Zinc released and distributed from Zinc oxide Nanoparticles in in vivo conditions in organs and blood of mice. As cost and availability of instrument is a...

07 July 2014 9,952 13 View