I am detecting singlet oxygen by DPBF using RoseBengal as standard, ethanol as solvent. Does DPBF/RB and Samples have a fixed concentration tobe used?

I have couple of queries related to SINGLET OXYGEN detection by DPBF: On what basis the concentration for DPBF/ Rose Bengal and samples are fixed?Do they (RB/DPBFand samples) have a fixed...

05 June 2016 5,720 1 View

Is there any standard procedure for removing yeast contamination from cryo preserved caco2 cell line before growing them?

I have cryopreserved caco2 cell line and the previous batch of cells had yeast contamination when cultured. Is there any procedure/washing that can be followed to remove the yeast contamination...

11 December 2014 6,301 7 View

Do Folate receptors express well in late passage of Caco2 cells?

I am working on Caco2 cells and interested in the folate receptors which are highly expressed in the cells. I am using a late passage (90), where cells are growing fine, but can someone...

11 December 2014 4,570 2 View

Can anyone share your experience of how stable nanoparticles were in different mediums over time?

10 November 2014 7,778 9 View

Can anyone provide me information about UV lamp and UV chamber suppliers, reasonable in cost within Australia?

I am in need of UV lamps (UVA/UVB/UVC) and UV chamber that can be used inside a biosafety cabinet for cell culture studies. The lamp needs to be compact and the chamber needs to be a closed one,...

08 September 2014 3,004 2 View

Does anyone know about distributors/designs of a UV chamber that can be used for both in vitro (cell culture) and in vivo (animal) studies?

I will be using UV radiation as part of my work in cell culture and animal studies. I would like to know if there is an UV chamber/design available that can be used for both purposes.

08 September 2014 7,901 5 View

Can anyone help me in designing a safe method for UV light exposure(from lamp) to cell cultures?

I need a safe procedure as it involves radiation. I am working on PDT, which involves UV light exposure to cell cultures. I need to know a safe, simple procedure to do the same. The source of...

07 August 2014 7,941 3 View

1,3-diphenylisobenzofuran(DPBF) is used to quantify singlet oxygen production, can we perform the same procedure in cell lines also?

07 August 2014 1,570 5 View

Is it possible to determine the amount of Zinc present in the blood of mice other than ICP-AES/ ICP-MS techniques

I am planning to determine the amount of Zinc released and distributed from Zinc oxide Nanoparticles in in vivo conditions in organs and blood of mice. As cost and availability of instrument is a...

06 July 2014 9,911 13 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View