I am detecting singlet oxygen by DPBF using RoseBengal as standard, ethanol as solvent. Does DPBF/RB and Samples have a fixed concentration tobe used?

I have couple of queries related to SINGLET OXYGEN detection by DPBF: On what basis the concentration for DPBF/ Rose Bengal and samples are fixed?Do they (RB/DPBFand samples) have a fixed...

05 June 2016 5,720 1 View

Is there any standard procedure for removing yeast contamination from cryo preserved caco2 cell line before growing them?

I have cryopreserved caco2 cell line and the previous batch of cells had yeast contamination when cultured. Is there any procedure/washing that can be followed to remove the yeast contamination...

11 December 2014 6,301 7 View

Do Folate receptors express well in late passage of Caco2 cells?

I am working on Caco2 cells and interested in the folate receptors which are highly expressed in the cells. I am using a late passage (90), where cells are growing fine, but can someone...

11 December 2014 4,570 2 View

Can anyone share your experience of how stable nanoparticles were in different mediums over time?

10 November 2014 7,778 9 View

How to detect singlet oxygen, superoxide radical by diphenylisobenzofuran (DPBF)?

I will be using DPBF for singlet oxygen detection, caused by nanoparticles in my study. Anyone who has used the method or followed a reference article, could you please provide me the details,...

09 October 2014 6,235 2 View

Can anyone provide me information about UV lamp and UV chamber suppliers, reasonable in cost within Australia?

I am in need of UV lamps (UVA/UVB/UVC) and UV chamber that can be used inside a biosafety cabinet for cell culture studies. The lamp needs to be compact and the chamber needs to be a closed one,...

08 September 2014 3,004 2 View

Does anyone know about distributors/designs of a UV chamber that can be used for both in vitro (cell culture) and in vivo (animal) studies?

I will be using UV radiation as part of my work in cell culture and animal studies. I would like to know if there is an UV chamber/design available that can be used for both purposes.

08 September 2014 7,901 5 View

1,3-diphenylisobenzofuran(DPBF) is used to quantify singlet oxygen production, can we perform the same procedure in cell lines also?

07 August 2014 1,570 5 View

Is it possible to determine the amount of Zinc present in the blood of mice other than ICP-AES/ ICP-MS techniques

I am planning to determine the amount of Zinc released and distributed from Zinc oxide Nanoparticles in in vivo conditions in organs and blood of mice. As cost and availability of instrument is a...

06 July 2014 9,911 13 View

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02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View