Putative Identity of this fungi?

I have been isolated from wheat disease. Among several isolates this isolate produce these kind of spores.

03 April 2018 7,949 6 View

Dear Colleagues what may be the identity of this fungi?

This pathogen has been isolated from wheat blast disease symptoms in Bangladesh. Isolate is cultured onto PDA media and two weeks age. I am confuse about its identity.Spores are septate Expecting...

02 March 2018 2,509 7 View

IAA extraction, purification and quantification protocol from rice seedling?

Please suggest me IAA extraction and determination protocol from rice.

02 March 2018 3,222 6 View

What is the putative identity of these fungal isolates?

Dear colleagues I have gotten these isolates from soil sample while trying to get entomopathogenic fungi. all are slow grower but produce numerous spores.

09 October 2017 8,206 11 View

Any biochemical test for Pyricularia?

To be sure, exlcuding PCR, is there any biochemical test or any kind of test for pyricularia? How spore production can be promoted for pyricularia? which media is good for maintenance and which...

04 May 2017 4,748 44 View

Anybody please suggest me the identity of this fungus?

04 May 2017 7,653 8 View

Which biochemical test I may perform to differentaite between Pseudomonas aeruginosa and Pseudomonas fluorescens?

I have few isolates that gave pigment on King's B. So which biochemical test I may do to be sure about P. fluorescens identity?

04 May 2017 6,580 24 View

Any selective media for isolating Pyricularia?

I used PDA and Swab cotton techniques to isolate Pyricularia but till now not successful. How may I start my job to isolate Pyricularia from rice sample?

04 May 2017 4,769 3 View

Identity of this fungi with clearer microscopic images, Neurospora fungi?

04 May 2017 3,573 15 View

How may I get Fusarium free phytophthora?

During Phytophthora culture I got fusarium contamination and its occurring every time. So which antibiotic or media or temperature I may use or maintain to get Fusarium free...

02 March 2017 8,823 16 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View