Hello,
I would like to detect viral infection in may plant tissue cultures. I know there is Elisa tests and RT-PCR methods but all of them are refered to one specific virus. Is there any methods to screen my plants and after positive outcome make decision about virus identification or to throw out same cultures. I would be grateful for any sugestions.
Dear Iwona
Virus isolation in cell cultures, immunofluorescence based assay and molecular techniques to determine nucleic acid, have all been used successfully to detect and identify the viruses. Since each of these established methods has several limitations, improved techniques of virus identification and quantification, such as mass spectrometry and next-generation sequencing/metagenomics are explored to overcome their limitations.
https://www.labome.com/method/Methods-for-Rapid-Virus-Identification-and-Quantification.html
It might be a good idea to pose this question directly to Scientia Terrae (http://www.scientiaterrae.org/). They have (I believe PCR based) arrays for plant pathogens.
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