Dear all,
Does anyone know the protocol for the best bacterial DNA isolation from raw milk? I would like to perform a full NGS identification of lactic acid bacteria and am looking for the best kit or procedure. Does anyone have experience with such research? Raw milk (brestmilk) hasn't got a large microbiome, hence it is not possible to collect bacteria by microfiltration. I've found a few articles about it, but I'm still looking for researchers who have any experience with such research. Anyone?
ultrafilterize the raw milk from fats and proteins.
acid precipitation and centrifugation for fats. and special starin for casein proteins. most bacteria should be with the watery part that leavesthe rest of the fats and proteins.
What we used to do is it suspend around 100uL into 900 of buffer or water and then homogenize it.
Afterwards, centrifuge at 10,000G and collect 400uL with the pellet inside.
- Badges
- Science method