Do you have any tips for antigen retrieval protocols with brain slices?

Dear All, I've been trying to stain brain slices with Anti-phospho-DARPP-32 (pThr34) SAB4504378 antibody with very poor results. The best were with antigen retrieval, but I have a problem with...

06 September 2020 6,974 3 View

What is tehe best method for magnolia rooting in in vitro cultures?

29 December 2019 1,472 4 View

What are most effective methods to teach SEN students communicate effectively?

I work as a lecturer in HCUC, London, in PMLD department. I am interested in new technology, tools, methods -that can support my students in learning to communicate effectively.

27 September 2019 2,408 4 View

What is the source of high frequency artifact inductive loop in a simple Three-Electrode System?

Could anyone help to solve my problem? I have recently investigated mesopourous layers of TiO2 on FTO in water electrolytes (just simple, known measuremensts) and I always observed an inductive...

17 January 2019 4,450 1 View

Are you still looking for contributions?

Hi, are you still looking for contributions? I'm researching informal economics in Dagestan, working on a project called "Anthropological study of corruption in Daghestan", quite in line with...

01 January 1970 4,743 2 View

Any news about the symphosium and the workshop?

Dear Glen I would like to ask if you are still oranizing the symphosium about cyanophyta and workshop about bcs? I have a project about cyanobacteria in microbial mats and soil crusts in hot...

01 January 1970 3,825 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View