Hi there Mahdi Ebadi,

Most vitamins (if not all of them) are degraded under high temperatures. I strongly recommend you start using filters with 0.22-micrometer pores. Perhaps you could prepare a stock solution, sterilize and distribute it in small flasks and keep them in a domestic freezer. Add them to the culture medium after it autoclave and temperatures around 50-60 ºC.

I know it is time-consuming, but your results would be secure.

It is better to filter by a sieve, it cannot have an impact on them.