12 December 2013 1 7K Report

Does it penetrate skin or show any allergenic reaction.

More Chandra Kishore's questions See All
What are the good journals in cancer field which don't charge for publication??

I want to know about the journals which don't charge for publication of an article and also the one which takes less time in processing.

03 April 2017 7,009 4 View

What concentration of TGF beta and TNF alpha should i use in SW480 and SW620 cell lines for pathway induction

I want to study the effect of drug on TGF beta and NFkB pathways in SW480 and SW620 cell lines.

11 December 2016 8,161 2 View

How many times can we use the same ?

1- 3% BSA blocking ? 2- Primary antibody 3- HRP conjugated secondary antibody 4- Transfer buffer 5- Running buffer

04 May 2016 1,060 3 View

What are the different grades of water used in molecular biology work.How we determine which grade to be used in what experiment ?

Milli Q, single distilled, double distilled, autoclaved, deionized - these are the words i encounter before water.I confuse a lot to understand these words.Is there any way to quickly determine...

10 November 2015 288 1 View

How many passages of colon cancer and cervical cell line can be maintained in the same dish or flask continuously without affecting the assays?

After a few passages I am getting change in morphology. When I had changed the serum it had also shown some changes in cell proliferation and growth pattern.

09 October 2015 4,526 11 View

Is there any effect of change in serum on cancer cell line?

I am observing some change in growth pattern and morphology

09 October 2015 3,943 5 View

Why should we run SDS-PAGE on constant voltage and Western Blot transfer on constant current ?

Protocols disagree on the  exact voltage and current set up for the above technique

11 December 2014 10,243 8 View

Does dmso of different grades make a difference in phytochemical drug solubility? Which is the best grade to solubilize phytochemical drug?

My drug is getting precipitated.

09 October 2014 10,162 6 View

Hesperidin is getting precipitated - How can I solve this problem?

I need hesperidin conc of 100,200,300,400 microM.I am doing following - Made 100 mM hesperidin in DMSO. Then took 1 micro L (100mM hesperidin) + 1000 micro L media i.e 100 microM. 2microL(100mM...

08 September 2014 8,907 6 View

Can anyone tell me is it OK to use 0.6 % highest conc. of DMSO in MTT for SW 620 and SW 480 cell lines?

I am able to get my ic50 only at this conc., 0.1% is not giving ic 50 at conc. of drug shown in literature.

06 July 2014 6,249 6 View

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