Bhairavi Srinageshwar

27 Questions 34 Answers 0 Followers

Questions related from Bhairavi Srinageshwar

Why is it that always the mutant protein is synthesized?

In autosomal dominant disorders I understand that the expression of one allele is required for the proper functioning. In case of mutations why not the protein from the normal allele get...

01 January 2015 4,417 6 View

Cell Culture and confluency ?

Usually how many days does it take for mesenchymal stem cells to attain 80-90% confluency?

11 November 2014 2,057 1 View

Protocol for culturing mouse iPSC

Can somebody share protocol for culturing mouse iPSC?

11 November 2014 8,926 3 View

Why is methionine always the first amino acid?

Why does tRNA recognize ATG as the first codon?

06 June 2014 2,331 11 View

Incorporation of ddGTP?

Why does ddGTP gets incorporated faster than other three ddNTPs during sequencing PCR?

05 May 2014 7,512 0 View

Is there any online tool to analyse frameshift mutation?

05 May 2014 2,167 6 View

Which is better for GC rich amplification: Betaine or DMSO?

Which is better for GC rich amplification?

04 April 2014 1,850 9 View

What is the difference between physical distance and genetic distance ?

04 April 2014 9,797 5 View

Can somebody share their GC rich kit protocol for PCR amplfication of difficult templates?

see above

02 February 2014 7,643 5 View

Why does the gel turn cloudy white as it gets solidified during a Native PAGE protocol?

see above

02 February 2014 9,114 9 View

How do some regions of the gene (or DNA) escape evolution and become conserved?

Thanks in advance.

02 February 2014 5,445 6 View

Why is the acceptor arm of tRNA always CCA3'?

01 January 2014 2,895 2 View

Why are intron exon boundaries always AG, while exon introns boundaries are always GT?

01 January 2014 9,328 7 View

Formula for calculating Tm of primers?

What are the different available formulae for calculating Tm of primers?

12 December 2013 8,647 13 View

What is Touch Down PCR ? When is it used?

12 December 2013 4,239 6 View

What is non-coding RNA and how does it play a role in disease?

Can somebody suggest a paper/article regarding this?

12 December 2013 1,292 4 View

What is basic, salt adjusted, and nearest neighbor when calculating the melting temperature for primer?

As given by some primer designing websites.

12 December 2013 3,781 8 View

Can somebody suggest a website from where I can download Free E Books on Medical genetics and neuroscience ?

12 December 2013 907 4 View

Can somebody suggest a protocol for DNA extraction from amniotic fluid ?

12 December 2013 5,411 4 View

What is the difference between the mind and the brain?

Where do my thoughts come from?

11 November 2013 7,071 20 View

Differential expression of the X chromosome in Brain

Why there is a differential expression of the X chromosome in brain ? How does it affect normal brain function?

11 November 2013 2,442 0 View

Albert Einstein's Brain

Why is Albert Einstein's Brain is of much interest in the field of research ? How was it different from other brains?

11 November 2013 1,297 4 View

Is being genius, a genetic trait

Is being genius, a genetic trait ?

11 November 2013 1,772 3 View

Imprinting and Epigenetics

Can somebody explain about Imprinting and Epigenetics ? How does it play a role in genetic disorders ?

11 November 2013 1,606 0 View

Are introns really a "JUNK" ?

Are non coding regions/introns in the human genome really a junk? Does it have any function?

11 November 2013 1,708 2 View

Did life evolve from RNA world?

11 November 2013 8,764 1 View

Difference in band intensity in agarose gel - can anyone help?

Why does the band intensity of 2 different products of similar size in agarose gel differ when the same concentration of DNA is used for PCR amplification? The condition used for PCR is also the same.

10 October 2013 7,557 10 View