Hello Everyone,
I am looking for a site where I can find metaphases with structural abnormalities of the chromosomes. I have some but their quality is not good enough for teaching.
Can any one help me?
Dear Mona
Please check this site, I hope benefit for you
Best wishes
Amin
https://www.genome.gov/11508982/chromosome-abnormalities-fact-sheet/
I think the two sites suggested would be of good help in providing reasonable examples for teaching chromosome abnormalities. However, it is important to understand that abnormalities that involve very small fragments of chromosome material may not be captured i.e may not be visible in such chromosome images.
Are you looking for GTG banded chromosomes for karyotypic abnormalities, or the Giemsa stained metaphase chromosomes for breaks, gaps, etc. structural abnormalities which are scored for genotoxicity assessment?
In either case, on-screen learning should be not without lot of microscopy as a base. Cytogenetics community knows what havoc can be played when sub-optimal chromosome preparations are observed using sub-optimal optics (and now CCD camera, and the computer screen)! At each level one can loose the image quality a lot. Hence, better to focus first on fine art and science of obtaining good quality metaphase [almost consistently]. The AGT manual book can help in wet-lab. The Plan Apo 100X objective, and good pixel rich CCD camera can give good images provided metaphases are good, well spread, and stained well.
By chance I came across following article today. This is worth reading also for the references given for training.
"European Journal of Human Genetics (2017) 25, 273–274; doi:10.1038/ejhg.2016.177; published online 21 December 2016"
Thank you guys. I needed a website with metaphases with G-banding, provided for learning so I can print it and give it to my students for the purpose of learning. They work under the microscope as well.
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