Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View

Can you help me choose a topic to my thesis?

Good Morning, I am a Ph.D. student studying " Information Management & Libraries" interested to work on a topic related to technology and how it is changing the future of libraries, but I...

14 December 2020 3,848 14 View

I need to know my H-index, my citations and publications?

How can I obtain my H-index, citations, publications?

01 December 2020 7,589 6 View

Why I am losing the Zona of pellocida while doing mouse preimplementation embryo staining?

I am doing some staining experiments in mice embryos and I noticed that I lose the zona of pellocida during the procedures. I can not define at exactly which step I lose it but mostly either...

15 October 2020 2,906 2 View

How to prepare 0.2 M Sodium Acetate Buffer Solution, pH 3.5?

12 October 2020 9,558 3 View

How to preserve Fe3O4@SiO2@Chitosan nanoparticles for a long time?

Is freeze-drying an appropriate method for these types of nanoparticles? If it's ok, what are the relevent buffers and conditions?

11 October 2020 2,276 3 View

Do you know of any source to buy hemp seeds?

Industrial hemp seeds with a good germination rate

28 September 2020 7,568 3 View

Is there a Deep Learning baseline (preferably written in PyTorch) for predicting animal pose from scratch (not like DeepLabCut or DeepPoseKit)?

I am looking for a animal pose estimation code that predicts the pose of an animal given 100-200 annotated frames from scratch using deep learning on a frame-by-frame basis. Is there any such...

27 September 2020 8,957 3 View

How to get Databases for MENA region banks?

I need to collect databases for Banks in the MENA region for my study, is there any public database or other databases that are affordable to access? Thank you.

30 August 2020 5,669 9 View

What is information system, data mining journal to publish survey/literature review paper ?

Your suggestion for an information system, data mining journal for publishing survey/literature review paper

13 March 2020 4,720 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

Can anyone suggest me broad host range fluorescence expression vectors for Gram-positive bacteria ?

Hi everyone, I am looking for fluorescence expression vectors for expression in gram-positive bacteria present in the skin microbiome. I need to tag them with a fluorescence marker and observe...

06 February 2021 2,566 4 View