I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And high PDI. Anyone has experience using extruder for this purpose?
Aggregation and agglomeration after extrusion caused by attractive van der Waals forces.
Mona Mirzaeimilani
You should have samples of micelles and cell membranes (extracted from cells). Remember that micelles and cell membranes need to be in water, as they self-assemble in water through hydrophobic interactions. The resulting mixture of micelles and membranes (two suspensions) is then extruded 10-20 times through a porous polycarbonate membrane of 400 nm thickness using an Avanti mini-extruder. Such mechanical force created by extrusion facilitates the fusion (self-assembly) of nanoparticles (micelles) with membranes. The consultant gets the impression that you are trying to put the extruder membrane on the micelles.
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