I obtained up to 30% encapsulation efficiency by using the desolvation synthesis method, in different pH level(7-8/5) with 8% glutaraldehyde as a cross-linker, this work has been repeated many times, but the amount of drug loading is low and has low repeatability.
Hello dear researcher
May you clarify What is your desolvating agent ? on top of that you may change it to another, or have u thought of the molecular size of doxorubicin since GA is a fantastic cross linking agent but the final structure would probably have size smaller than the drug molecule I guess. Personally I found better results using ECH( epichlorohydrin).
Desolvating agent that I applied is ethanol 99%
Doxorubicin is smaller than whole of BSA nanocarrier ( 1.5 nm beside 100_300 nm)
Thanks for your reply, Ok so I guess there is a misunderstanding in the second part, what I meant was sth else, desolvating process is a fantastic yet tricky method which seeks monitoring for each and every factors involved in your method let me elaborate by an example, since this method at the end relies on the difference of the gelation parameters like, the desolvating agent and the momentum of it's addition into the gel, the concentration of the BSA and the drug, the amount of cross linker used, the pH of the medium And etc. has an overt relation to the size and drug loading efficiency as I recommended some cross linkers showed good results since they are better in entrapment of the drugs glucose is one when speaking of BSA nano carriers if I'm not mistaken the abbreviation, for your first question as I mentioned you may do a literature search to determine if other researchers have observed the same amount of loading with doxorubicin, other than that try to change the so called crucial factors for higher drug uptake I believe that since glutaraldehyde bounds with the free amine in the surface of BSA directly impacts the surface charge other cross linkers such as glucose and sorbitol and ECH are different, for the think that your results are not repeatable all I can say is that in your case some of these factors might been varied simultaneously since a small shift in the pH would affect drug intake, the concentration of the protein used if changed the particle size and agglomeration would impact drug dissociation, adjusting the interfering factors is really a concern.
Please feel free to get in touch if there are questions or the response is not clear
Best Wishes
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