Which search engines can I use to get the source of a certain microbial strain?

I am searching for a certain website through which can I get the source of certain microbial strains, especially probiotic strains I mean a search engine contains a database of the identified...

20 December 2020 5,144 5 View

Can you help me choose a topic to my thesis?

Good Morning, I am a Ph.D. student studying " Information Management & Libraries" interested to work on a topic related to technology and how it is changing the future of libraries, but I...

14 December 2020 3,848 14 View

I need to know my H-index, my citations and publications?

How can I obtain my H-index, citations, publications?

01 December 2020 7,589 6 View

Why I am losing the Zona of pellocida while doing mouse preimplementation embryo staining?

I am doing some staining experiments in mice embryos and I noticed that I lose the zona of pellocida during the procedures. I can not define at exactly which step I lose it but mostly either...

15 October 2020 2,906 2 View

How to prepare 0.2 M Sodium Acetate Buffer Solution, pH 3.5?

12 October 2020 9,558 3 View

How to preserve Fe3O4@SiO2@Chitosan nanoparticles for a long time?

Is freeze-drying an appropriate method for these types of nanoparticles? If it's ok, what are the relevent buffers and conditions?

11 October 2020 2,276 3 View

Do you know of any source to buy hemp seeds?

Industrial hemp seeds with a good germination rate

28 September 2020 7,568 3 View

Is there a Deep Learning baseline (preferably written in PyTorch) for predicting animal pose from scratch (not like DeepLabCut or DeepPoseKit)?

I am looking for a animal pose estimation code that predicts the pose of an animal given 100-200 annotated frames from scratch using deep learning on a frame-by-frame basis. Is there any such...

27 September 2020 8,957 3 View

How to get Databases for MENA region banks?

I need to collect databases for Banks in the MENA region for my study, is there any public database or other databases that are affordable to access? Thank you.

30 August 2020 5,669 9 View

What is information system, data mining journal to publish survey/literature review paper ?

Your suggestion for an information system, data mining journal for publishing survey/literature review paper

13 March 2020 4,720 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

I am looking for PCR primers for bacillus Sp.

15 February 2021 2,552 5 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View