20 Questions 57 Answers 0 Followers
Questions related from Fatma Abdel Wahab
I am using Trizol reagent for RNA Extraction This is nanodrop evaluation after 1/10 dilution Is this curve accepted
05 April 2023 792 3 View
Iam using Trizol for RNA expression experiment Here is the concentration and Purity Any recommendations for enhancing the concentration and purity How to eliminate phenolic contamination that...
24 January 2023 6,068 4 View
Can any one tell me if we can use multiple stem loops primers in the same RT reaction (sybrbgreen)?
19 December 2022 1,187 1 View
Had any one used Total RNA extraction kit for micro RNA extraction
25 October 2021 7,100 3 View
How many can I store EDTA blood samples in - 20 before genomic DNA extraction?
07 August 2021 4,484 3 View
I have a submitted a manuscript The status was for two month under review then turned awaiting reviewer selection
01 June 2020 901 3 View
what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...
02 December 2018 7,147 1 View
Hi all I am new in the field of medical statistics, I want a link to vedios on how to perform meta-analysis
03 August 2018 6,180 3 View
hellow all When to use Delta CT vs DD CT in gene expression assay
26 January 2018 7,652 2 View
Hi all any one knowa how to export FCS files. from Beckman Coulter Navious ?
07 September 2017 4,354 3 View
Hi all I want to ask about the diffrence between LMD files and FCS files in using asotware for flowcytometry analysis
05 September 2017 2,317 2 View
Hi all I amworking on LncRNA gene expression after amplification the house keeping gene give CT about 19- 22 But my gene of interest give very high CT above 40 What? should I do
11 August 2017 305 0 View
I was doing a mutation screening experiment using meltdoctor master mix. 18 cases. In two cases no flurcence was observed any explaination? NB these cases was used in another expriment and were...
06 August 2017 1,557 4 View
Has any one tried RNA extraction from ,72 hours old bone marrow sample EDTA blood
01 May 2017 5,984 5 View
I use QuiaCube automated RNA extraction from PB MNCs??
27 March 2017 9,669 4 View
RNA extraction
28 February 2017 5,848 2 View
Some cases of refractory AML are given low doses of chemotherapy , would that affect their gene expression profiling ?
04 February 2017 6,879 2 View
annimal.studies on Bne marrow biopsy
23 January 2017 1,143 1 View
For a case of acute leukemia
13 January 2017 9,749 4 View
I sent a manuscript for publish in one of Willey journals since March 24 , the status is saying under review for 2 month till now ,how to proceed
01 January 1970 6,832 8 View