Fatma Abdel Wahab

20 Questions 57 Answers 0 Followers

Questions related from Fatma Abdel Wahab

Phenolic or trizol contamination?

I am using Trizol reagent for RNA Extraction This is nanodrop evaluation after 1/10 dilution Is this curve accepted

05 April 2023 790 3 View

Low purity RNA?

Iam using Trizol for RNA expression experiment Here is the concentration and Purity Any recommendations for enhancing the concentration and purity How to eliminate phenolic contamination that...

24 January 2023 6,067 4 View

Stem loop primers?

Can any one tell me if we can use multiple stem loops primers in the same RT reaction (sybrbgreen)?

19 December 2022 1,186 1 View

Can total RNA extraction kit extract the MI-RNA From plasma?

Had any one used Total RNA extraction kit for micro RNA extraction

25 October 2021 7,099 3 View

Dna extraction from frozen tissues?

How many can I store EDTA blood samples in - 20 before genomic DNA extraction?

07 August 2021 4,483 3 View

What is meant by awaiting reviewer selection after being under review in scholar one online submission system?

I have a submitted a manuscript The status was for two month under review then turned awaiting reviewer selection

01 June 2020 899 3 View

Paired samples statistics?

what is the minmum count to perform paired sample gene expression assay if my cases have pre and post reading eg I have 4 cases with low expression and 6 cases of high expression baseline and...

02 December 2018 7,146 1 View

How to perform meta-analysis ?

Hi all I am new in the field of medical statistics, I want a link to vedios on how to perform meta-analysis

03 August 2018 6,179 3 View

Delta CT in qPCR Reactions?

hellow all When to use Delta CT vs DD CT in gene expression assay

26 January 2018 7,651 2 View

Navious. flow cytometry ?

Hi all any one knowa how to export FCS files. from Beckman Coulter Navious ?

07 September 2017 4,353 3 View

Flow cytometry analysis ?

Hi all I want to ask about the diffrence between LMD files and FCS files in using asotware for flowcytometry analysis

05 September 2017 2,316 2 View

Gene expression ?

Hi all I amworking on LncRNA gene expression after amplification the house keeping gene give CT about 19- 22 But my gene of interest give very high CT above 40 What? should I do

11 August 2017 304 0 View

No flurecence at one PCR well?

I was doing a mutation screening experiment using meltdoctor master mix. 18 cases. In two cases no flurcence was observed any explaination? NB these cases was used in another expriment and were...

06 August 2017 1,556 4 View

RNA extraction from 72 hour old sample?

Has any one tried RNA extraction from ,72 hours old bone marrow sample EDTA blood

01 May 2017 5,982 5 View

How long I can preserve RNA extract in -20?

I use QuiaCube automated RNA extraction from PB MNCs??

27 March 2017 9,668 4 View

How to overcome the low WBCs count while RNA extraction from PB MNCs?

RNA extraction

28 February 2017 5,847 2 View

Do any one know if the refractory AML cases gene EXPRESSION profiling is affected by subcutanous low dose AraC as a palliative treatment?

Some cases of refractory AML are given low doses of chemotherapy , would that affect their gene expression profiling ?

04 February 2017 6,878 2 View

What about if I forgot a bone marrow biopsy specimens in NBF for 15 days, for research purpose ?

annimal.studies on Bne marrow biopsy

23 January 2017 1,141 1 View

I have an EDTA blood sample,that ACD was added to it as a preservative for flowcytometry , can I use it for DNA and RNA extraction ?

For a case of acute leukemia

13 January 2017 9,748 4 View

Long time peer review

I sent a manuscript for publish in one of Willey journals since March 24 , the status is saying under review for 2 month till now ,how to proceed

01 January 1970 6,831 8 View