Can anyone recommend me a ELISA TGF-beta 1 kit to test human serum samples?

I would like to quantify the systemic levels of TGF-B1 in human patients. My previous experience was with Biolegend in primary human cells but I'm not finding clinical papers that used the kit...

07 August 2014 7,796 10 View

Does anyone know what rat tissues can be used as positive control for BMP-2 IHC?

I am trying to perform anti-BMP-2 IHC in formalin-fixed rat femur using Abcam primary antibody (ab14933). The manual suggests spleen, lung and intestine as positive controls but until now I didn't...

06 July 2014 5,553 1 View

How can osteoblasts be detected in histological slides from formalin-fixed paraffin-embedded rat femurs?

I would like to evaluate new bone formation in response to a biomaterial. TRAP (Sigma kit) seems to be a good option for osteoclasts but I am not finding a histochemistry method for osteoblasts...

03 April 2014 9,458 5 View

Can anyone recommend me an anti-Rat Integrin Beta 2 (CD18) antibody to perform immunohistochemistry on formol-fixed or paraffin-embedded bone tissues?

I am looking for leukocyte infiltration at a bone defect/biomaterial interface. At the lab, we have just a WT.3 clone antibody-FITC conjugated certified to FACS analysis. I am planning to try it...

02 March 2014 9,447 1 View

Which house keeping genes can I use to normalize the expression of genes of interest in Real Time-qPCR using human synovial tissues?

I am studying immune reactions in human synovial tissues and I am planning to analyze gene expression. A former student has used B2M as housekeeping gene but I am not sure if it is the most...

09 October 2013 2,212 28 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View