AAS is pointed as a good tool to assess the magnesium content of a solution. However, I would like to know if there is an easier way of determine the levels of magnesium.
I would like to quantify the systemic levels of TGF-B1 in human patients. My previous experience was with Biolegend in primary human cells but I'm not finding clinical papers that used the kit...
07 August 2014 7,847 10 View
I am trying to perform anti-BMP-2 IHC in formalin-fixed rat femur using Abcam primary antibody (ab14933). The manual suggests spleen, lung and intestine as positive controls but until now I didn't...
06 July 2014 5,593 1 View
I would like to evaluate new bone formation in response to a biomaterial. TRAP (Sigma kit) seems to be a good option for osteoclasts but I am not finding a histochemistry method for osteoblasts...
03 April 2014 9,499 5 View
I am looking for leukocyte infiltration at a bone defect/biomaterial interface. At the lab, we have just a WT.3 clone antibody-FITC conjugated certified to FACS analysis. I am planning to try it...
02 March 2014 9,493 1 View
I am studying immune reactions in human synovial tissues and I am planning to analyze gene expression. A former student has used B2M as housekeeping gene but I am not sure if it is the most...
09 October 2013 2,267 28 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View