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Questions related from Daniel Marques Vasconcelos
I would like to quantify the systemic levels of TGF-B1 in human patients. My previous experience was with Biolegend in primary human cells but I'm not finding clinical papers that used the kit...
08 August 2014 7,832 10 View
I am trying to perform anti-BMP-2 IHC in formalin-fixed rat femur using Abcam primary antibody (ab14933). The manual suggests spleen, lung and intestine as positive controls but until now I didn't...
07 July 2014 5,577 1 View
I would like to evaluate new bone formation in response to a biomaterial. TRAP (Sigma kit) seems to be a good option for osteoclasts but I am not finding a histochemistry method for osteoblasts...
04 April 2014 9,483 5 View
I am looking for leukocyte infiltration at a bone defect/biomaterial interface. At the lab, we have just a WT.3 clone antibody-FITC conjugated certified to FACS analysis. I am planning to try it...
03 March 2014 9,475 1 View
I am studying immune reactions in human synovial tissues and I am planning to analyze gene expression. A former student has used B2M as housekeeping gene but I am not sure if it is the most...
10 October 2013 2,249 28 View
AAS is pointed as a good tool to assess the magnesium content of a solution. However, I would like to know if there is an easier way of determine the levels of magnesium.
11 November 2012 6,259 0 View