Longest culture time for intracellular cytokine staining?

I want to incubate human CD4 T memory cells for 48 to dterminate cycle entry and proliferation. I plan to stimulate them for 48 hours. Can I add brefeldin for the last hors of this incubation in...

05 June 2019 612 4 View

Is making a start-up the only way to transfer technology?

My Institution just applied for a patent based on a finding of my group (we are based in Mexico). I want to promote its licensing to some company that might be interested. Has someone done this?...

10 November 2018 7,627 7 View

What kit for intracellular NAD determination do you recommend?

I need to measure intracellular NAD (total and NAD+) in human T cells. I see there are several systems (colorimetric and ELISA), and I need a kit that is reliable. I don't want to use HPLC for...

07 August 2018 7,150 1 View

CD4+ T cell lines expressing and not expressing CD38?

08 September 2017 9,020 2 View

What is the difference between Myc and cMyc tag proteins?

We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know...

08 September 2016 2,345 4 View

What online resources do you recommend for teachin immunology?

I am teaching a general immunology undergraduate course to students of laboratory medicine and pharmacology. I am looking for images, diagrams, text, and things of the sort as a support to my classes.

07 August 2016 833 3 View

What is the best LPS to induce inflammation in mice by ip injection?

LPS is obtained from different bacteria, and their activities may differ. Additionally the degree of glycosilation also influences LPS activity. Which origin or brand or kind of LPS yields a clear...

04 May 2016 2,058 6 View

Are there accepted markers of cell-cycle arrest?

We are looking for molecules whose presence is unique to arrest in G2 or M phases of cells cycle, whether as a result or as a cause of this arrest. We need to detect a molecule evidencing arrest...

31 December 2014 319 7 View

Can the maximal expression in an experiment be used as reference delta cT to calculate deltaDelta cT (Instead of the basal expression)?

10 November 2014 3,139 5 View

What's best to determine cell division (proliferation) by FACS, CFSE or PKH26?

I want to decide which dye to use to detect actual mitosis (not just DNA syntesis or cycling) in a highly purified subset of T cells. Does anyone has experience using both CFSE and PKH26? What's...

08 September 2014 7,830 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View