We need to make a tough decision. We need a good sorter, contained in a Class I/A2 biosafety cabinet. I would appreciate if you share your experience. Is it better than BD's influx?
i think for S2 ( biosafety cabinet)lab the Facs Aria III is the best
I want to incubate human CD4 T memory cells for 48 to dterminate cycle entry and proliferation. I plan to stimulate them for 48 hours. Can I add brefeldin for the last hors of this incubation in...
05 June 2019 799 4 View
My Institution just applied for a patent based on a finding of my group (we are based in Mexico). I want to promote its licensing to some company that might be interested. Has someone done this?...
10 November 2018 7,788 7 View
I need to measure intracellular NAD (total and NAD+) in human T cells. I see there are several systems (colorimetric and ELISA), and I need a kit that is reliable. I don't want to use HPLC for...
07 August 2018 7,316 1 View
Do Jurkat and other CD4 T cell lines express CD38? Ideally I would like to have a CD38neg and a CD38pos line. I got lost in the ATCC web page trying to find subclones of the main cell lines. I...
08 September 2017 9,205 2 View
We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know...
08 September 2016 2,526 4 View
I am teaching a general immunology undergraduate course to students of laboratory medicine and pharmacology. I am looking for images, diagrams, text, and things of the sort as a support to my classes.
07 August 2016 1,063 3 View
LPS is obtained from different bacteria, and their activities may differ. Additionally the degree of glycosilation also influences LPS activity. Which origin or brand or kind of LPS yields a clear...
04 May 2016 2,237 6 View
We are looking for molecules whose presence is unique to arrest in G2 or M phases of cells cycle, whether as a result or as a cause of this arrest. We need to detect a molecule evidencing arrest...
31 December 2014 502 7 View
We measured the induction of a series of genes in purified subsets of T cells cells, by polyclonal stimulation through the TCR. Since the conditions varied slightly between experiments, we...
10 November 2014 3,329 5 View
I want to decide which dye to use to detect actual mitosis (not just DNA syntesis or cycling) in a highly purified subset of T cells. Does anyone has experience using both CFSE and PKH26? What's...
08 September 2014 7,997 6 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View