Longest culture time for intracellular cytokine staining?

I want to incubate human CD4 T memory cells for 48 to dterminate cycle entry and proliferation. I plan to stimulate them for 48 hours. Can I add brefeldin for the last hors of this incubation in...

05 June 2019 612 4 View

Is making a start-up the only way to transfer technology?

My Institution just applied for a patent based on a finding of my group (we are based in Mexico). I want to promote its licensing to some company that might be interested. Has someone done this?...

10 November 2018 7,627 7 View

CD4+ T cell lines expressing and not expressing CD38?

08 September 2017 9,020 2 View

What is the difference between Myc and cMyc tag proteins?

We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know...

08 September 2016 2,345 4 View

What online resources do you recommend for teachin immunology?

I am teaching a general immunology undergraduate course to students of laboratory medicine and pharmacology. I am looking for images, diagrams, text, and things of the sort as a support to my classes.

07 August 2016 833 3 View

What is the best LPS to induce inflammation in mice by ip injection?

LPS is obtained from different bacteria, and their activities may differ. Additionally the degree of glycosilation also influences LPS activity. Which origin or brand or kind of LPS yields a clear...

04 May 2016 2,058 6 View

Are there accepted markers of cell-cycle arrest?

We are looking for molecules whose presence is unique to arrest in G2 or M phases of cells cycle, whether as a result or as a cause of this arrest. We need to detect a molecule evidencing arrest...

31 December 2014 319 7 View

Can the maximal expression in an experiment be used as reference delta cT to calculate deltaDelta cT (Instead of the basal expression)?

10 November 2014 3,139 5 View

What's best to determine cell division (proliferation) by FACS, CFSE or PKH26?

I want to decide which dye to use to detect actual mitosis (not just DNA syntesis or cycling) in a highly purified subset of T cells. Does anyone has experience using both CFSE and PKH26? What's...

08 September 2014 7,830 6 View

Can phosphatidylserine be exposed by live cells not undergoing canonical apoptosis?

Is it possible that cells identified as apoptotic by their viability plus Annexin V binding are actually undergoing a process of cell death or cycle arrest different to canonical apoptosis? For...

06 July 2014 2,873 14 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

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03 March 2021 5,360 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View