I need to measure intracellular NAD (total and NAD+) in human T cells. I see there are several systems (colorimetric and ELISA), and I need a kit that is reliable. I don't want to use HPLC for the moment.
This may be helpful....
I want to incubate human CD4 T memory cells for 48 to dterminate cycle entry and proliferation. I plan to stimulate them for 48 hours. Can I add brefeldin for the last hors of this incubation in...
05 June 2019 799 4 View
My Institution just applied for a patent based on a finding of my group (we are based in Mexico). I want to promote its licensing to some company that might be interested. Has someone done this?...
10 November 2018 7,788 7 View
Do Jurkat and other CD4 T cell lines express CD38? Ideally I would like to have a CD38neg and a CD38pos line. I got lost in the ATCC web page trying to find subclones of the main cell lines. I...
08 September 2017 9,205 2 View
We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know...
08 September 2016 2,526 4 View
I am teaching a general immunology undergraduate course to students of laboratory medicine and pharmacology. I am looking for images, diagrams, text, and things of the sort as a support to my classes.
07 August 2016 1,063 3 View
LPS is obtained from different bacteria, and their activities may differ. Additionally the degree of glycosilation also influences LPS activity. Which origin or brand or kind of LPS yields a clear...
04 May 2016 2,237 6 View
We are looking for molecules whose presence is unique to arrest in G2 or M phases of cells cycle, whether as a result or as a cause of this arrest. We need to detect a molecule evidencing arrest...
31 December 2014 502 7 View
We measured the induction of a series of genes in purified subsets of T cells cells, by polyclonal stimulation through the TCR. Since the conditions varied slightly between experiments, we...
10 November 2014 3,329 5 View
I want to decide which dye to use to detect actual mitosis (not just DNA syntesis or cycling) in a highly purified subset of T cells. Does anyone has experience using both CFSE and PKH26? What's...
08 September 2014 7,997 6 View
Is it possible that cells identified as apoptotic by their viability plus Annexin V binding are actually undergoing a process of cell death or cycle arrest different to canonical apoptosis? For...
06 July 2014 3,048 14 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...
02 August 2024 3,174 1 View
Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...
31 July 2024 6,464 5 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...
28 July 2024 7,827 6 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...
26 July 2024 5,654 3 View
I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...
24 July 2024 9,799 3 View