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Questions related from Enrique Espinosa
I want to incubate human CD4 T memory cells for 48 to dterminate cycle entry and proliferation. I plan to stimulate them for 48 hours. Can I add brefeldin for the last hors of this incubation in...
06 June 2019 706 4 View
My Institution just applied for a patent based on a finding of my group (we are based in Mexico). I want to promote its licensing to some company that might be interested. Has someone done this?...
11 November 2018 7,721 7 View
I need to measure intracellular NAD (total and NAD+) in human T cells. I see there are several systems (colorimetric and ELISA), and I need a kit that is reliable. I don't want to use HPLC for...
08 August 2018 7,244 1 View
Do Jurkat and other CD4 T cell lines express CD38? Ideally I would like to have a CD38neg and a CD38pos line. I got lost in the ATCC web page trying to find subclones of the main cell lines. I...
09 September 2017 9,112 2 View
We are going to clone a protein in a pCMV-Myc plasmid. Looking for fluorochrome-conjugated antibodies to track the efficiency of transfection, I found both cMyc and Myc antibodies. I don't know...
09 September 2016 2,447 4 View
I am teaching a general immunology undergraduate course to students of laboratory medicine and pharmacology. I am looking for images, diagrams, text, and things of the sort as a support to my classes.
08 August 2016 966 3 View
LPS is obtained from different bacteria, and their activities may differ. Additionally the degree of glycosilation also influences LPS activity. Which origin or brand or kind of LPS yields a clear...
05 May 2016 2,152 6 View
We are looking for molecules whose presence is unique to arrest in G2 or M phases of cells cycle, whether as a result or as a cause of this arrest. We need to detect a molecule evidencing arrest...
01 January 2015 418 7 View
We measured the induction of a series of genes in purified subsets of T cells cells, by polyclonal stimulation through the TCR. Since the conditions varied slightly between experiments, we...
11 November 2014 3,240 5 View
I want to decide which dye to use to detect actual mitosis (not just DNA syntesis or cycling) in a highly purified subset of T cells. Does anyone has experience using both CFSE and PKH26? What's...
09 September 2014 7,924 6 View
Is it possible that cells identified as apoptotic by their viability plus Annexin V binding are actually undergoing a process of cell death or cycle arrest different to canonical apoptosis? For...
07 July 2014 2,965 14 View
We are analyzing memory CD4 T cell responses (cytokines, co-stimulatory molecules and chemokine receptors) before and after TCR-mediated stimulation. GAPDH gave nice parallel efficiencies with the...
09 September 2013 3,605 13 View
I am measuring IL-2, interferon gamma, IL-17 and a number of other genes in memory CD4 T cells after polyclonal activation with anti-CD3. I am using expression of GAPDH as a housekeeping (after...
09 September 2013 2,595 1 View
I'm analyzing flow data with FloJo. The little square left of the sample id, whose color indicates the compensation matrix utilized, turned filled, instead of only gridded and I cannot analyze it...
06 June 2013 9,173 3 View
What is signal width (i.e. ssc-w) used for? I use height (FSC-H) plotted against FSC-A (area) to gate singlets, then analyzing PBMCs or T cells but I see there is the option of w for FSC and SSC....
05 May 2013 5,026 5 View
We need to make a tough decision. We need a good sorter, contained in a Class I/A2 biosafety cabinet. I would appreciate if you share your experience. Is it better than BD's influx?
03 March 2013 9,348 1 View
I am going to use Click-iT Edu reagent and protocol to detect cell proliferation by flow cytometry, on cells staining for extracellular and intracellular proteins. The reagents are compatible with...
03 March 2013 4,655 14 View
Edu: 5-ethynyl-2’-deoxyuridine, a thymidine analogue, allows to detect DNA synthesis (S-phase) by using a reaction that makes incorporated Edu fluorescent, and thus, amenable to flow cytometry....
02 February 2013 4,100 10 View
I need to sort cells (PBMCs mainly) from patients with untreated HIV, active tuberculosis and pandemic H1N1. So I need the cytometer to fit into a Class II Type A2 Biological Safety Cabinet or...
10 October 2012 1,594 4 View
