We normally use 4X protein sample lysis buffer containing 250 mM Tris (pH 6.8), 40% glycerol, 10% β-mercaptoethanol, 9.2% SDS, 0.02% bromophenol blue. I have used 2-D quant kit (GE, Health care) for protein quantification for 2D samples (which normally doesn't contain glycerol, bromophenol etc).

I was just wondering if someone has used this kit with SDS-PAGE sample preparation buffer (as described above) which contains glycerol and bromophenol blue (two things which 2-D kit doesn't claim any tolerance or compatibility with, otherwise excellent in presence of SDS, CHAPS or other constituents of buffers).

I want to use this 2-D kit for quantification of my protein samples which are normally prepared in sample lysis buffer as described above but has never used this kit for these samples. Any suggestion or experience regarding this would be highly appreciated.