Muhammad Akbar Shahid

5 Questions 6 Answers 0 Followers

Questions related from Muhammad Akbar Shahid

How can we determine the concentration of antibiotic pellet available with cartridge?

How can we determine the concentration of antibiotic pellets available with the antibiotic cartridge? For example, Meropenem concentration from Oxoid is 10 ug per disc, but what would be the...

23 September 2021 2,757 3 View

Is it compulsory to first use HRM calibration kit for performing HRM on CFX96 Real Time machine?

To perform HRM analysis for genotyping on CFX96 machine for the first time, BioRad recommends to use HRM calibration kit along with Precision melt analysis software. I am just wondering whether...

09 December 2015 4,868 13 View

Is it still possible to compare growth of different bacterial strains with slightly different inoculum concentration?

For example: if one strain has starting inoculum (CCU/ml) of log10 3.5, second with 4 and third with 3, is it still possible to measure and quantitate the difference in their growth rate? If yes...

08 July 2014 7,627 5 View

Is it OK to use 2-D quant kit with protein sample lysis buffer containing glycerol and Bromophenol blue?

We normally use 4X protein sample lysis buffer containing 250 mM Tris (pH 6.8), 40% glycerol, 10% β-mercaptoethanol, 9.2% SDS, 0.02% bromophenol blue. I have used 2-D quant kit (GE, Health care)...

31 May 2014 7,292 3 View

How to normalize whole cell protein concentration using SDS-PAGE for different strains of a bacteria for which no internal control is available?

I want to determine relative expression of a particular protein by immunostaining/ western blot, using Diaminobenzidine (DAB) method, in mycoplasma cells for which, as such, no internal control is...

29 May 2014 9,664 3 View