I do not know exactly the concentration of cell is 5x10^5 or 6x10^4 - 8x10^4.
First of all what kind of cell do you grow? it depends on the cell tipe but generally 6,25X10^5 will be ok.
Generally speaking, the area of a single well in the 24 well plate is 1 in 5 compared with the 6 well plate, and 1 in 30 compared with 10 cm dish. So as for adherent cells, let's say HeLa cell for example, I always plate 1.5*10^6 cells for 10cm dish to reach about 30% confluence, so I guess I would plate 5*10^4 cells for a single well in the 24 well plate.
Hi Hoa Ky Lan
As the growth area in each 24 well plate is 1.9 cm2.
So, the total growth surface- area is 1.9*24 = 45.6 cm2.
While the surface area of the 6 cm2 plate equals 21 cm2.
Suppose that you have 80 % confluence and you need to split the cells to 10 % confluence.
80 to 10
So, the answer is 1/8
You want to transfer cells from 21 cm2 to 45.6 cm2
which equals 1 : 2
# of cells/ surface area = 1/2
So you need 1/2 * ? = 1/8
The answer is 1/4
So, 1/4* # cells in the 6 cm2 should be divided over the area of 24 well plate.
Best regards
You can transfer cells by calculation of surface area and confluence of the cells or you could try seeding it at a definite cell number. The purpose of the experiment will usually determine the cell number or confluence.
Usually, a 25% confluent plate is good enough for an experiment to be done within 2 days. Based on the confluence of the starting culture vessel, surface area of the starting vessel, required confluency in your experimental well and surface area of your experimental well and your purpose, you could find the split ratio as mentioned by Gaber Ramadan in this post.
Although you could consider the suggestions given herein, it would be best if you worked out the conditions yourself based on the necessity of the experiment and the length of the experiment. Good luck
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