Until now, I already have 3 fragments of TRPM2, so, how I can connect 3 fragments to each other? How can I insert it into the pCI Neo vector?
Run an overlap PCR and clone it into the pCI Neo Vector.
Now, Im working on using CRISPR tool to knock out TRPM2 gene in zebrafish. Is there any easy way to identify which is homo fish and which is hetero fish?
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I want study the role of TRPM2 to animals immune system and seeking advice on the topic
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kindly reply me. Thanking you in advance.
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