How to identify KO zebrafish?

Now, Im working on using CRISPR tool to knock out TRPM2 gene in zebrafish. Is there any easy way to identify which is homo fish and which is hetero fish?

31 December 2017 3,119 3 View

In cell culture, when we transfer from 6 cm dish to 24 well plate, how much concentration of cell we use?

I do not know exactly the concentration of cell is 5x10^5 or 6x10^4 - 8x10^4.

09 October 2013 9,688 8 View

Could you please tell me, which concentration I should choose when I want to find IC50 of Pyr2?

Using Pyr2 concentration in Ca2+ imaging. I want to find out IC50 of Pyr2 from 0uM to 10uM.

09 October 2013 3,464 3 View

What is the role of ATP in the Ca 2+ imaging protocol?

I use ATP in the Ca2+ imaging, but something I do not know, what ATP play the role in the process of Ca2+ imaging? I do not know why, when I add ATP into dish, I saw some cells become disappear....

09 October 2013 5,429 2 View

What's the difference between the upper and lower buffers in western blotting?

Why do we use different pH levels for the upper and lower buffers (upper 6.8 and lower 8.8). What's the difference between them?

09 October 2013 4,332 2 View

What is the role of TRPM2 in animals immune system?

I want study the role of TRPM2 to animals immune system and seeking advice on the topic

09 October 2013 7,244 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View