I want study the role of TRPM2 to animals immune system and seeking advice on the topic
Now, Im working on using CRISPR tool to knock out TRPM2 gene in zebrafish. Is there any easy way to identify which is homo fish and which is hetero fish?
31 December 2017 3,192 3 View
Until now, I already have 3 fragments of TRPM2, so, how I can connect 3 fragments to each other? How can I insert it into the pCI Neo vector?
03 April 2014 8,422 1 View
I do not know exactly the concentration of cell is 5x10^5 or 6x10^4 - 8x10^4.
09 October 2013 9,759 8 View
Using Pyr2 concentration in Ca2+ imaging. I want to find out IC50 of Pyr2 from 0uM to 10uM.
09 October 2013 3,538 3 View
I use ATP in the Ca2+ imaging, but something I do not know, what ATP play the role in the process of Ca2+ imaging? I do not know why, when I add ATP into dish, I saw some cells become disappear....
09 October 2013 5,494 2 View
Why do we use different pH levels for the upper and lower buffers (upper 6.8 and lower 8.8). What's the difference between them?
09 October 2013 4,407 2 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...
07 August 2024 2,569 1 View
I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.
06 August 2024 6,796 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View