How to identify KO zebrafish?

Now, Im working on using CRISPR tool to knock out TRPM2 gene in zebrafish. Is there any easy way to identify which is homo fish and which is hetero fish?

31 December 2017 3,119 3 View

How can I connect 3 fragments with pCI Neo vector?

Until now, I already have 3 fragments of TRPM2, so, how I can connect 3 fragments to each other? How can I insert it into the pCI Neo vector?

03 April 2014 8,349 1 View

In cell culture, when we transfer from 6 cm dish to 24 well plate, how much concentration of cell we use?

I do not know exactly the concentration of cell is 5x10^5 or 6x10^4 - 8x10^4.

09 October 2013 9,688 8 View

Could you please tell me, which concentration I should choose when I want to find IC50 of Pyr2?

Using Pyr2 concentration in Ca2+ imaging. I want to find out IC50 of Pyr2 from 0uM to 10uM.

09 October 2013 3,464 3 View

What is the role of ATP in the Ca 2+ imaging protocol?

I use ATP in the Ca2+ imaging, but something I do not know, what ATP play the role in the process of Ca2+ imaging? I do not know why, when I add ATP into dish, I saw some cells become disappear....

09 October 2013 5,429 2 View

What's the difference between the upper and lower buffers in western blotting?

Why do we use different pH levels for the upper and lower buffers (upper 6.8 and lower 8.8). What's the difference between them?

09 October 2013 4,332 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View