Ha Nam Tran

7 Questions 13 Answers 0 Followers

Questions related from Ha Nam Tran

How to identify KO zebrafish?

Now, Im working on using CRISPR tool to knock out TRPM2 gene in zebrafish. Is there any easy way to identify which is homo fish and which is hetero fish?

01 January 2018 3,162 3 View

How can I connect 3 fragments with pCI Neo vector?

Until now, I already have 3 fragments of TRPM2, so, how I can connect 3 fragments to each other? How can I insert it into the pCI Neo vector?

04 April 2014 8,396 1 View

What's the difference between the upper and lower buffers in western blotting?

Why do we use different pH levels for the upper and lower buffers (upper 6.8 and lower 8.8). What's the difference between them?

10 October 2013 4,376 2 View

In cell culture, when we transfer from 6 cm dish to 24 well plate, how much concentration of cell we use?

I do not know exactly the concentration of cell is 5x10^5 or 6x10^4 - 8x10^4.

10 October 2013 9,728 8 View

What is the role of ATP in the Ca 2+ imaging protocol?

I use ATP in the Ca2+ imaging, but something I do not know, what ATP play the role in the process of Ca2+ imaging? I do not know why, when I add ATP into dish, I saw some cells become disappear....

10 October 2013 5,470 2 View

Could you please tell me, which concentration I should choose when I want to find IC50 of Pyr2?

Using Pyr2 concentration in Ca2+ imaging. I want to find out IC50 of Pyr2 from 0uM to 10uM.

10 October 2013 3,513 3 View

What is the role of TRPM2 in animals immune system?

I want study the role of TRPM2 to animals immune system and seeking advice on the topic

10 October 2013 7,282 0 View