Siyuan Su

11 Questions 40 Answers 0 Followers

Questions related from Siyuan Su

Can GSEA (Gene Set Enrichment Analysis) be applied to a proteomics dataset?

Most of proteomic researchers abstract the biological significance of their proteomic data by examine the up/down-regulated proteins first then perform functional enrichment analysis on these...

02 February 2015 8,419 3 View

Can we export all the measured data (FCS, SSC, FITC, TRITC) for a single cell to the excel table?

I am using an Aria from BD. I want to export all the data for all the single cells to excel or txt for data analysis, not just the histogram or scatterplot. Is it possible? How to do that?

02 February 2014 9,620 2 View

Is acridine orange a proper marker for lysosomes?

My purpose was to demonstrate the lysosomal permeabilization using acridine orange. I read a few papers on the LMP, most of them suggested the red fluorescence of acridine orange is an indicator...

02 February 2014 9,089 3 View

Can anyone help with problems with statistical tests for detecting ROS using H2-DCFDA in flowcytometry?

I am trying to use flowcytometry to detect ROS using H2-DCF. Previously we have detected and compared the ROS level of treated and control cells using 96-well plate and the results are good, both...

11 November 2013 376 8 View

Can someone advise on whether to perform RNase first or RNase together with PI?

I am going to do DNA content analysis on HeLa cells and I searched for relevant protocols. Some of them suggest do PI staining and RNase A together, while others stated it was better to do RNase A...

10 October 2013 9,309 4 View

Is there any reliable software that can count cells based on image taken under a microscope?

Need to process large quantities of image. Please give me some advices. Thanks

07 July 2013 1,854 22 View

Help with 3D graphic representation of protein expression level.

I have a group of protein expression levels across three different conditions. What I want is to present the expression level of individual proteins across conditions as they are on X, Y and Z...

05 May 2013 1,779 1 View

Acridine orange for lysosomal permeability assay in flow cytometry

Anyone used acridine orange for lysosomal permeability assay in cytometry? Is there a detailed protocol for me to refer to?

02 February 2013 8,112 1 View

Artificial effect of doxycycline on protein oxidation and transcription factors

In our lab, we often use doxycycline to induce gene expression or knockdown whose expression was regulated by Tet-regulated systems. This system is quite powerful and efficient until I found...

01 January 2013 8,275 6 View

Trouble with cell counting on SA beta-gal staining

I am currently doing senesecence-related beta-gal staining. The problem is I have to spend a lot of time to count the cells, which was indeed countless. Does any one have an idea about how many...

11 November 2012 3,367 3 View

Does anybody do WB to detect c-Src abundance in cells? Do you consider its solubility?

I used to use buffer with 1% NP-40, 50mM Hepes and 150mM NaCl as my lysis buffer when analyzing Src protein abundance with my samples. Since c-Src is a membrane anchored protein, I am wondering if...

06 June 2012 8,828 2 View