José Diego Pignataro

4 Questions 19 Answers 0 Followers

Questions related from José Diego Pignataro

Is it possible to have negative regulatory elements in the intron sequence of a plasmid backbone that could cause a reduction in promoter activity?

I am quite sure that the answer is no, but if change the original promoter (lets say a CMVenh-CMV) for a neuronal promoter and I would like to identify the presence of negative regulators of...

08 August 2015 7,059 2 View

How much could mycoplasma affect an AAV production?

I was keeping HEK 293T cells for an AAV production, although my last mycoplasma test was positive. I already discard the cells in order to avoid more contamination in our culture room, but I would...

01 January 2015 1,523 8 View

Does anyone know a convenient and free software to detect binding sites for transcription factors?

I would like to perform an animal promoter characterization and genomic organization.

03 March 2014 6,856 0 View

Could polybrene affect lentivirus infection efficiency in HEK 293T cells?

I have performed an infection in 293T cells with a purified and concentrated LV stock (for GFP expression). One day after plate 293T cells in a p100, I prepared the medium (DMEM 10%FBS+Pen/Strep)...

02 February 2014 8,975 8 View