I'm facing irregular colony after transformation of Ecoli competent cell in LR reaction. I thought that there's problem with the antibiotics in the plate. However I tried to use new plate for transformation process, it showed the same.
There's details for my lab : 100ng entry vector pdonr221-gene (4500bp), 300ng destination vector pBGWFS7 (12700bp), TE buffer and LR clonase enzymes were mixed together, incubated overnight at 25. Proteinase K was added, incubated for 10min at 37. 5ul of reaction added into 50ul ecoli competent cell. Transformation process went as normal. Lastly, the plates were incubated at 37 overnight.
What do you mean by "irregular colony"? Do you mean that you think you have contamination with another type of bacteria? Your colonies look a bit "overgrown" - like you let them grow at 37* a bit too long which is why some of the colonies are very large. It's normal to have some size variation in E. coli colonies.
Unless I'm misunderstanding your question, it looks to me like your transformation was a success!
I think this plate was contaminated from different bacteria. I can see at least three different bacteria colony type in the plate. You should purify your bacteria before you make a test.
I am agree with others. You can select 10 colonies for plasmid extraction. Finally you can select the right one by PCR or restriction digestion.
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