I am about to do PAH analysis on crude oil containing my fungal samples. I have tried methods such as using hexane to separate my fungal samples from the oil but to no avail because the organic matter refused to separate from the oil.
I am faced with the situation of possibly doing PAH analysis without separating the fungi sample from the oil. I am not sure if the presence of my fungal samples will have any effect on the results of the PAH analysis or if the PAH analysis would work. The PAH analysis method I would be doing is the IP 391.
Caren,
if you will make extraction for example with hexane/dichloromethane and after that you will use 0,2 um filters - probably it will not be an issue.
regards,
GB
In addition to Grzegorz's answer, I would also 'spike' a sample and calculate the %Recovery.
I do not see how the presence of fungi will affect the concentration of PAH at the time of analysis. If the analysis is targeted at PAH concentration, then PAH you will get. I don't know of any method for PAH analysis that microbial component will interfere with. I do not think fractionation of petroleum takes into consideration, the microbial content of petroleum.
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