Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.
MIC can be determined by serial dilution method using 96 well microplate and indicator resazurin. first of all broth will be added to all the well used, then the drug (known concentration) will be added to the topmost well and will serially diluted downward, after that the bacterial strain will be added to each well equally, after that incubate it for 24 hrs, after 24 hrs indicator will be added to each well and wait for 60 minutes and observe the color change, if it turns pink then the last blue well be the MIC of the drug.
The Fractional Inhibitory Concentration (FIC) index is used to evaluate the interaction between two antimicrobial agents in combination. First of all the MICs of the drug alone and combination will be determined. After that FIC of each drug can be determined as
FIC of drug A FICA=MICA(combination)/ MICA
FIC of drug B: FICB=MICB(combination)/MICB
Then FIC index can be determined by the following formula
FIC index = FICA+FICB
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