Dear eminent researchers iam planning to start IHC study in ameloblastoma using FAP ALPHA. Need your opinion?

Fibroblast activation protein (FAP )alpha

28 December 2020 8,101 0 View

Which is the best machine learning method for topic extraction from large document collections?

I want to know about different algorithm/method/inference to extract underlying topics/themes from documents/scientific article etc.

12 October 2020 5,911 3 View

The porous nature of the material should show up in the impedance behaviour in terms of inductance element?

The porous nature of the material should show up in the impedance behaviour in terms of inductance element. I do not see any value given for the inductance at the interface in these plots which...

01 October 2020 7,504 2 View

Is there any way to separate MoS2 QDs from MoS2 flakes?

I have tried to exfoliate MoS2 via liquid exfolaition method(using NMP as well as Ethanol/water mixture as solvent) . Always Iam getting MoS2 nanoflakes and MoS2 QDs. Is there any way to separate...

03 February 2020 6,771 1 View

I want to coat Ionic liquid over Fe3o4@Sio2. I have very concentrated only 0.19g of ionic liquid. Does anyone have idea how can i do it?

I have successfully coated the Fe304@Sio2@PPy( Polypyrrole) simply by taking 100mg Fe304@Sio2 ,suspended in 15 ml DI water then sonicate for 15 min then add 100 microlitre pyrrole. Solution was...

13 January 2020 8,206 5 View

Wether it is possible to dissolve Amyloid beta 1-40 monomer with phosphate buffer or do we need to HFIP?

I have Amyloid beta 1-40 monomer using which i need to perform biophysical characterisations.

16 September 2019 4,472 3 View

Support vector machine or neural network, which techniques is better for image classification problem?

pattern classification is a popular problem area, there are different methods available to do it.

16 September 2019 3,549 3 View

How the sampling is done in cohort,case control and cross sectional design ?

how the sampling is done in cohort,case control and cross sectional design ?

14 August 2018 830 5 View

Data Required

Hello friends, I need to know how to get the data if you are researching on supply chain. I am looking for costs and other inventory data for retail chains of India. How to go about it?

25 May 2017 783 3 View

Instead of osmium tetroxide is there any other substance that can be used as a secondary fixative for electron microscopy of bacterial cell?

25 September 2016 9,374 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View