In reference to antibiotic as a commodity, what is your take and way forward on common molecules in both Veterinary and Human antibiotics in relation to currently experienced emergency of AMR?
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,726 6 View
Dunn's equation should not be used in the case of a battery-type electrode, according to a paper published in the journal "Phys. Chem. Lett. 2020, 11, 9846–9849 (ref. attached)" that I recently...
12 May 2024 3,260 0 View
Hello everyone,I am currently working with the multivariate time series forecasting by using NeuralProphet to predict the price of product.I used 7 economic indicators as regressor in my...
22 March 2024 8,264 3 View
I would like to know that prophet time series model is under the category of neural network or machine learning or deep learning? I want to forecast the price of product depending on other...
19 February 2024 1,241 1 View
Dear RG users, I hope all of you are doing well. I just wanted to know how can I do dispacement controlled cyclic triaxial tests using ELDYN dynamic equipment. As you can see in the figure, there...
14 September 2023 2,217 0 View
generally polar basis set will provide the tensors (dipole moment,polarizability and hyperpolarizability). but how to find their contributions separately
24 June 2023 1,294 6 View
To study the effects of cell conditioned medium (CM) in animals in vivo, is it necessary to concentrate the medium after collection? I am not familiar with the preparation of the CM.
01 March 2023 694 3 View
These two models take a lot of time to train with powerful GPUs and required a minimum 512 batch size for good model performance.
28 February 2023 1,653 1 View
Width of depletion region which can be remarked using width of space charge layer (WSCL).
06 February 2023 2,620 0 View
My research is about convert agricultural waste into food using food processing techniques. My existing objectives are production, cost analysis, and evaluation of product in terms of color,...
09 December 2022 2,452 3 View
Can someone explain to me how was the MIC of MO in combination and MIC of antibiotic in combination was calculated? as shown in the attached file below.
26 July 2024 2,701 1 View
I am on gene work to express gene of interest in some Bacillus species. Thus far, i need to try some RBS in Vector which is optimized for bacillus subtilis. Before i design vector to express in...
11 July 2024 7,846 1 View
I'm going to transfect cells with expression plasmids and later siRNAs against the same gene. Is it true that penicillin/streptomycin in the media negatively affects these processes? Shout I use...
07 July 2024 8,669 6 View
I read a paper where they converted WGS to core genome to check for AMR through resfinder, any insights would be appreciated, new to bioinformatics.
10 June 2024 8,347 1 View
Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?
10 June 2024 9,739 0 View
Hello, I recently started working with antibiotics and I do not have that much experience of making dilutions accurately. I want to ask if I suppose to add 200mg of antibiotic in 100ml of water...
08 June 2024 2,285 6 View
So I made a 100 mg/ml solution of Ampicillin and in order to solubilize the powder I used 1 M NaOH. I noticed the solution turns yellow shortly after dissolving and I was wondering if some...
05 June 2024 7,169 0 View
The antibiotic powder is dissolved in Milli-q water and then filtered using syringe filter for the analysis.
01 June 2024 7,418 0 View
I'm conducting protein-protein interaction studies using a LexA-based Bacterial-Two Hybrid system with E. coli reporter strains. We employ MacConkey agar and β-galactosidase assays to evaluate the...
26 May 2024 4,646 0 View
Hi all, I am wondering if anyone has any experience with using InvivoGen's Primocin antibiotic solution beyond the indicated expiry date when properly aliquoted and stored at -20degC. We have a...
20 May 2024 7,612 2 View