Technical site help needed: I need to change my log-in email address and can't get to the HELP section?

My old log in email, [email protected], was compromised and I need to change it to [email protected], so I can change my password, update publications, etc.

24 December 2020 6,939 3 View

Which Universities are virtual?

Many Universities have employees located across the world.

22 December 2020 6,380 3 View

How do I test for multicollinearity among Independent variables in binary logistic regression using R?

Please, I am trying to do a project work using logistic regression. I need to test for multicollinearity for the independent variables using R. I installed mctest and multiColl package already....

18 December 2020 2,531 3 View

Where can I find graphs of Brazilian Portuguese phonology?

I need research material on Brazilian Portuguese phonology.

27 September 2020 9,274 3 View

How to avoid getting organic particles to charr(become black) during calcination?

I am trying to synthesize SrHfO3:Eu2+ nanoparticles. To reduce from Eu3+ to Eu2+, we calcinate it under Ar/H2 conditions but it ends giving a black coloured product. We need this for luminescence...

22 August 2020 448 3 View

How does one collect data from a population that was once together on a university campus but is now dispersed due to the Covid-19 lockdowns??

If a researcher is more than half way through a research project, and now needs to collect data by recording the responses of participants to a read aloud test; How can such a researcher collect...

03 August 2020 6,771 5 View

How can we calculate polystyrene nanoparticles concentration in a solution?

I wonder how to measure the concentration of polystyrene nanoparticles (50nm) in a solution. Our nanoparticles are not fluorescents. Thanks

28 October 2019 2,353 4 View

I need some information on the method of manufacturing dry extract?

i need some information on the method of manufacturing dry extract?

06 February 2018 5,565 2 View

How can measure the concentration of dopamine and serotonin in rat brain tissue?

How can measure the concentration of dopamine and serotonin in rat brain tissue?

11 December 2017 6,461 10 View

How can I export my publications as a Bib TeX file

I want to post my publications on ORCID and I can't see how to save them as a Bib TeX file in order to export them, as the instructions on ORCID say.

29 June 2017 6,242 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View