There are 3 options for polymer dye buffers:

1. Super bright staining buffer

2. Brilliant stain buffer

3. Brilliant stain buffer plus (more concentrated version, which requires less volume)

One of these buffers should be used when using 2 or more polymer dyes to prevent dye-dye interactions. These buffers don't need to be included in single stain controls. Most compensation issues are due to poor single stains. If you have dim single stains on cells you should consider compensation beads.

I hope this helps. Happy flowing!