I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2 slides to reduce fluorescence.
I acquired an extended scan with 10s exposure time, and for some reason, many of the spectra don't seem to have acquired the entire spectrum, starting spectrum acquisition from a somewhat variable Raman shift.
All spectra are acquired from the same area of the tissue (no background spectra). I've attached some pictures of the raw spectra. Has anyone any idea what might be happening? Thank you.
Chris Taylor, the InVia system avoids a certain amount of light from hitting the detector by cutting the signal if the signal is higher than a certain threshold. So, you can try to decrease the exposure time and lower the laser power.
I hope this helps.
From your spectroscopy, it seems to me that the significant impact of noise baseline is not fluorescence but the laser source itself. The response of the detector, at the raman shift of zero, is from the laser itself. Hence, your detecor is saturated and cut off by the system to avoid the damage on yiur detector.
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