Mohamed Khashan

10 Questions 183 Answers 0 Followers

Questions related from Mohamed Khashan

What is the highest accepted error bar value?

Hi friends, While carrying out MTT assay, I encountered with a value of 6 for one error bar of the experiment groups. So, I would like to ask what is the highest accepted error value in...

12 July 2022 8,088 2 View

Unreadable results of Thioflavin S fluorescence?

Hi friends, Last week I carried out an experiment to detect the effect of an agent of interest on KCL-induced alpha synuclein aggregations in SH-SY5Y. We used three wells in a 24-well plate as...

16 June 2022 8,731 8 View

What is the reason of noisy background in IF and GFP photos?

Hi dear friends, I am working on Parkinson model. I transfected SH-SY5Y cells with topo isomerase IIB by lipofectamine 3000 reagent. I took some photos today but their backgrounds were too...

14 May 2022 488 3 View

How to calculate the efficiency of EGFP-tagged plasmid?

Hello Friends, In a research they calculated (by a microscope) the efficiency of EGFP-tagged plasmid, but they didn't mention the way of calculating. What is the possible ways to carry out this...

15 April 2022 8,152 4 View

Why the satin of alpha synuclein is over the nucleus?

Hello friends, We treated SH-SY5Y cells with 50 mM and 100 mM KCL to induce alpha synuclein accumulation. After 24 and 48 hours, we detected the aggregates of alpha synuclein by Zeiss...

09 April 2022 7,109 4 View

How can we prepare 50 mM KCL to depolarize cells and how to be added to the medium?

Hello friends, We are trying to depolarize neuronal cell by kcl, research used this way didn't elaborate much about preparing kcl. I read that we can prepare kcl medium, but I don't have a clear...

26 February 2022 3,734 4 View

What are differences between LC3A and LC3B antibodies, which used in autophagy?

Hello friends, While I was reviewing research related to autophagy activation, I noticed that there are two types of LC3 antibodies used to detect the activation of autophagy. Those two types...

19 February 2022 8,626 9 View

How can I replace eGFP found in alpha synuclein plasmid with mCherry?

We would like to use an enzyme tagged with eGFP and eGFP-tagged alpha synuclein at the same time to observe them under confocal microscopy. However, this will give wrong readings, so we would like...

07 November 2021 1,224 4 View

How to ask for a gift of alpha synuclein A53T lentivirus?

Hello Dears, I am trying to inspect the effect of an enzyme on alpha synuclein A53T related to Parkinson's disease (PD). Unfortunately, I am working without funder and I can't purchase it. I have...

01 January 1970 6,101 1 View

What are the best references for PCR

Hi, I am interested in learning more about technologies that focus on gene expression. Could you please recommend the best references to gain a solid understanding of the techniques related to...

01 January 1970 727 4 View