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Questions related from Mohamed Khashan
Hi friends, While carrying out MTT assay, I encountered with a value of 6 for one error bar of the experiment groups. So, I would like to ask what is the highest accepted error value in...
12 July 2022 8,077 2 View
Hi friends, Last week I carried out an experiment to detect the effect of an agent of interest on KCL-induced alpha synuclein aggregations in SH-SY5Y. We used three wells in a 24-well plate as...
16 June 2022 8,721 8 View
Hi dear friends, I am working on Parkinson model. I transfected SH-SY5Y cells with topo isomerase IIB by lipofectamine 3000 reagent. I took some photos today but their backgrounds were too...
14 May 2022 473 3 View
Hello Friends, In a research they calculated (by a microscope) the efficiency of EGFP-tagged plasmid, but they didn't mention the way of calculating. What is the possible ways to carry out this...
15 April 2022 8,140 4 View
Hello friends, We treated SH-SY5Y cells with 50 mM and 100 mM KCL to induce alpha synuclein accumulation. After 24 and 48 hours, we detected the aggregates of alpha synuclein by Zeiss...
09 April 2022 7,097 4 View
Hello friends, We are trying to depolarize neuronal cell by kcl, research used this way didn't elaborate much about preparing kcl. I read that we can prepare kcl medium, but I don't have a clear...
26 February 2022 3,717 4 View
Hello friends, While I was reviewing research related to autophagy activation, I noticed that there are two types of LC3 antibodies used to detect the activation of autophagy. Those two types...
19 February 2022 8,611 9 View
Hello Dears, I am trying to inspect the effect of an enzyme on alpha synuclein A53T related to Parkinson's disease (PD). Unfortunately, I am working without funder and I can't purchase it. I have...
01 January 1970 6,090 1 View
