Please tell me the best PBS recipe for both cell culture and super resolutionmicroscopy?

When ever I mount the cover slips on the slides to perform the microscopy I find crystals on the other side of the cover-slip after drying. please suggest me the better recipe where i can use it...

04 May 2019 4,263 5 View

Can any one suggest me the best ratios for fugene transfection with HEK293 cells?

after transfection with fugene reagent i can see most cells are dying. please suggest me a good practice protocol to get best transfection and better ratios. need regular method used in the lab.

01 February 2019 9,084 2 View

How to check endoplasmic reticulam morphology during stress condetions ?

I want to check ER morphology during stress conditions, which enhances cell death, can any one suggest me any better and simple method. and I also want to check ATG release from the cells?

03 April 2017 8,119 2 View

Do you want to participate in national symposium on current research in cancer biology & therapy?

06 July 2016 8,129 0 View

Why do we use wbc chamber in hemocytmeter for cancer cell lines count when ever we split the cell why dont we use RBC chamber?

give me a better answer only size is the major reason or is there any other restrictions or can we use RBC chamber also.

06 July 2016 5,685 2 View

Is there any evidence that animal plant cell fusion and successful revival?

because i want to know regarding plant cytoplasam and animal cytoplasam differences?

06 July 2016 5,103 1 View

Why do we use methanol in western blotting? and why do we use methanol for pvdf membrene activation?

please answer me with detailed chemical reaction. thanking you.

06 July 2016 366 4 View

Can anyone provide me with a definition of liveliness?

We are trying to propose the relation between animal psychological behavior with it's DNA for my dram project. Regarding this I've produces me own definition liveliness. Can anyone provide me with...

02 March 2015 906 4 View

What is livingness? How it differs in a moving architecture and non moving architecture of living thing?

i need some details to confer it. please share and help me

01 January 1970 4,008 0 View

Which type of material used for making animal cell culture plates ? which material able adhere to with animal cells growth factors?

I need the material used in making and how that material can form bond with growth factors.

01 January 1970 3,328 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

03 March 2021 5,360 2 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View