I want to know the required setup for establishing T cell culture in my mammalian cancer cell line lab, at less cost. please suggest me the better way to start the things.
Whether you are looking to perform the co culture experiments between cancer cells and T cells.
When ever I mount the cover slips on the slides to perform the microscopy I find crystals on the other side of the cover-slip after drying. please suggest me the better recipe where i can use it...
04 May 2019 4,348 5 View
after transfection with fugene reagent i can see most cells are dying. please suggest me a good practice protocol to get best transfection and better ratios. need regular method used in the lab.
01 February 2019 9,197 2 View
I want to check ER morphology during stress conditions, which enhances cell death, can any one suggest me any better and simple method. and I also want to check ATG release from the cells?
03 April 2017 8,197 2 View
Hi friends do you like to participate in national symposium on current research in cancer biology & therapy? Here is the attachment and links please go through it. It’s a nice one.
06 July 2016 8,231 0 View
give me a better answer only size is the major reason or is there any other restrictions or can we use RBC chamber also.
06 July 2016 5,771 2 View
please answer me with detailed chemical reaction. thanking you.
06 July 2016 442 4 View
because i want to know regarding plant cytoplasam and animal cytoplasam differences?
06 July 2016 5,179 1 View
We are trying to propose the relation between animal psychological behavior with it's DNA for my dram project. Regarding this I've produces me own definition liveliness. Can anyone provide me with...
02 March 2015 997 4 View
i need some details to confer it. please share and help me
01 January 1970 4,092 0 View
I need the material used in making and how that material can form bond with growth factors.
01 January 1970 3,396 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View