I need the material used in making and how that material can form bond with growth factors.
let me see If I understood... mammalian cells usually stick on plastic surfaces (there are many class of polymers), however, to increase the adherence (especially for primary cultures) other attachment agents are used (poly-lysine, gelatin, collagen, etc). Now , if you want to put some growth factors on this surface matrix, I don see any problem if you dilute some factors on gelatin solution.for instance... then you can apply this solution to the plaques and seed the cells once this film has dried .
If you want a better answer, then you are going to have to put more effort into your question. Please provide more specific details about what you are trying to do.
i need the chemical material to make the plate. because the ErbB1 over express and activated by ligands and form bonding with the surface. it should form bonding with particular components only, as Omar Porras mentioned poly-lysine, gelatin, collagen, are there on the surface of the plate and and the receptor binds to it. so, My question is what is the material or chemical used in making the plate can binds to the receptor, event that collagen, poly-lysine, gelatin also binds to that that i want to know, wether it is forming any hydrophobic bond, vanderval force which type of chemical reaction taking place with that surface?
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