Please tell me the best PBS recipe for both cell culture and super resolutionmicroscopy?

When ever I mount the cover slips on the slides to perform the microscopy I find crystals on the other side of the cover-slip after drying. please suggest me the better recipe where i can use it...

04 May 2019 4,263 5 View

Can any one suggest me the best ratios for fugene transfection with HEK293 cells?

after transfection with fugene reagent i can see most cells are dying. please suggest me a good practice protocol to get best transfection and better ratios. need regular method used in the lab.

01 February 2019 9,084 2 View

How to check endoplasmic reticulam morphology during stress condetions ?

I want to check ER morphology during stress conditions, which enhances cell death, can any one suggest me any better and simple method. and I also want to check ATG release from the cells?

03 April 2017 8,119 2 View

Do you want to participate in national symposium on current research in cancer biology & therapy?

06 July 2016 8,129 0 View

Why do we use wbc chamber in hemocytmeter for cancer cell lines count when ever we split the cell why dont we use RBC chamber?

give me a better answer only size is the major reason or is there any other restrictions or can we use RBC chamber also.

06 July 2016 5,685 2 View

Is there any evidence that animal plant cell fusion and successful revival?

because i want to know regarding plant cytoplasam and animal cytoplasam differences?

06 July 2016 5,103 1 View

Can anyone provide me with a definition of liveliness?

We are trying to propose the relation between animal psychological behavior with it's DNA for my dram project. Regarding this I've produces me own definition liveliness. Can anyone provide me with...

02 March 2015 906 4 View

What is livingness? How it differs in a moving architecture and non moving architecture of living thing?

i need some details to confer it. please share and help me

01 January 1970 4,008 0 View

Which type of material used for making animal cell culture plates ? which material able adhere to with animal cells growth factors?

I need the material used in making and how that material can form bond with growth factors.

01 January 1970 3,328 3 View

I want to establish t cell culture in my cancer cell lines lab can any one suggest me the better and good way to start my work?

01 January 1970 8,312 1 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View